摘要
目的建立小儿腹泻外敷散丁香酚和桂皮醛的含量测定方法。方法采用高效液相色谱法,色谱柱：Eclipse XDB-C18柱,流动相：甲醇-水（65∶35）,紫外检测波长：280nm,流速：1.0mL.min^-1。结果丁香酚在9.635-154.16μg范围内线性关系良好（r=1.0000）;平均回收率为99.5%,RSD=1.56%。桂皮醛在2.74-43.76μg.mL^-1范围内线性关系良好（r=1.0000）;平均回收率为100.0%,RSD=0.65%。结论该方法简便、准确,可用于制剂的质量控制。
OBJECTIVE To establish a RP-HPLC method for determination of Eugenol and Cinnamaldhyde in Xiao er Fuxie Waifu powder.METHODS C18 column was adopted as stationary phase,solvent mixiure composed of methanol-water（65∶35） as mobile phase,the detection wavelength was 280nm.RESULTS Eugenol had a good linearity（r=1.000 0）in the range of 9.635～154.16μg·mL^-1.The average recovery was 99.5%,RSD was 1.56%;Cinnamaldhyde had a good linearity（r=1.0000） in the range of 2.74～43.76μg·mL^-1.The average recovery was 100.0%,RSD .was 0.65 %. CONCLUSION The above method is simple, sensitive and accurate, and maybe used for the quality control.
出处
《海峡药学》
2009年第10期45-46,共2页
Strait Pharmaceutical Journal
关键词
小儿腹泻外敷散
丁香酚
桂皮醛
高效液相色谱
Xiao er Fuxie Waifu powder
Eugenol
Cinnamaldhyde
High performance liquid chromatography
