摘要
目的:对薏苡仁水煎液进行雌激素样活性筛选,探讨其作用机制。方法:首先通过小鼠子宫增重实验和人乳腺癌细胞(MCF-7)增殖实验对薏苡仁水煎液进行雌激素样活性筛选;以质粒p ERE-TAL-luc、pβgal-Control、p CXN2-h ERα或p CXN2-h ERβ共转染的HEK293细胞株为模型,采用以ERE调控的报告基因luc瞬时表达检测技术,评价薏苡仁水煎液雌激素样作用的信号通路。结果:薏苡仁水煎液可显著的诱导性未成熟小鼠子宫系数的增加(P<0.01),且在去雌激素的培养基中能够显著促进MCF-7细胞的增殖(P<0.01);ERE调控的报告基因瞬时表达结果显示,分别由ERα和ERβ介导时,与正常组相比,薏苡仁水煎液的标准化荧光素酶活性明显升高(P<0.05或P<0.01)。结论:薏苡仁水煎液可诱导性未成熟小鼠子宫系数的增加,在去雌激素的培养基中诱导MCF-7人乳腺癌细胞的增殖,首次发现薏苡仁具有雌激素样作用,且主要是通过ERβ介导发挥雌激素样作用。
This study was aimed to investigate the estrogenic effects ofCoicis Semen in order to preliminarily discuss the mechanism. Mouse uterine weight test and MCF-7 cell proliferation assay were used to evaluate the estrogenic effects ofC. Semen. Reporter gene assays were adopted to explore the action mechanism ofC. Semen. In reporter gene assay, HEK293 cells were co-transfected with pERE-TAL-luc, pβgal-Control, pCXN2-hERα, or pCXN2-hERβ. And the expression of reporter gene luc was controlled by ERE. Mouse uterine weight test showed that compared with the control group, the aqueous extracts ofC. Semen can increase the uterus index of premature female mice （P〈0.01）. It can significantly promote the proliferation of MCF-7 cells in the medium without estrogen （P〈0.01）. The reporter gene controlled by ERE technology showed thatwhen mediated by ERα or ERβ respectively, the normalized luciferase activity of aqueous extracts ofC. Semen was significantly higher than activity of the control group （P〈0.05 orP〈0.01）. It was concluded that the aqueous extracts ofC.Semen can increase the uterus index of premature female mice and promote the proliferation of MCF-7 cells in the medium without estrogen. We found the estrogenic effects ofC. Semen for the first time. And the estrogenic effects ofC. Semen were mainly mediated by ERβ.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第9期1974-1979,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
科学技术部国家重点基础研究发展计划"973计划"项目(2013CB531802):宣泻利水中药的药性研究
负责人:郑晓珂
