摘要
[目的]建立评价金银花抗氧化活性的方法,比较不同产地金银花药材抗氧化活性大小,确定抗氧化活性成分的贡献率。[方法]采用在线快速活性筛选高效液相色谱-二极管阵列检测器-1,1-二苯基-2-苦基肼(HPLC-DAD-DPPH)甲醇枸橼酸在线体系,建立金银花抗氧化活性指纹图谱。[结果]确定了绿原酸和异绿原酸A为其主要的抗氧化活性成分,以金银花中8个主要活性成分为抗氧化活性指标对不同产地金银花的质量进行控制,确定了广州野生金银花质量最佳。[结论]该方法简便可行,基于活性整合指纹图谱技术筛选药材中的抗氧化活性成分并以活性成分为指标,为全面有效评价中药质量提供了可借鉴的模式。
[Objective] To establish a method for evaluating the antioxidant activity of Flos Lonicerae, comparing antioxidant activity of Flos Lonicerae from different origins and determine the contribution rate of the each active antioxidant ingredient. [Methods] Using an active filter with fast online system, HPLC-DAD-1,1- diphenyl-2-picrylhydrazyl （HPLC-DAD-DPPH） in methanol citrate online system, the antioxidant activity fingerprints of Flos Lonicerae was established. [Results] Identifying the main antioxidant active ingredients and using chlorogenic acid and ehlorogenic acid A, the quality control of Flos Lonicerae in different origins was accomplished taking eight main active ingredients in Flos Lonicerae as the indicators of antioxidant activity. It was demonstrated that the quality of the wild Flos Lonicerae from Guangzhou was the best amang all selected samples. [Conclusion] This method is simple and feasible. It is based on activity integrated fingerprint technology for screening the active antioxidant ingredients in herb and as an index, providing a reference model for evaluation of the quality of traditional Chinese medicine comprehensively and effectively.
出处
《天津中医药大学学报》
CAS
2014年第5期291-295,共5页
Journal of Tianjin University of Traditional Chinese Medicine
基金
国家自然基金青年基金资助项目(81001632)
