摘要
目的研制酶联免疫吸附试验试剂盒中各组分的稳定剂,使得试剂盒保存期达到国家相关规定。方法采用经验法和正交法相结合的办法,配制一系列稳定剂配方,对酶标板、酶标抗体及标准品等进行处理,通过对照试验,筛选出稳定效果最好的配方。结果筛选到一种包被稳定剂(质量分数:牛血清清蛋白0.5%,明胶0.25%,海藻糖5%,PEG4000 0.1%),一种酶标抗体稳定剂(质量分数:牛血清清蛋白1%,蛋白胨1%,蔗糖10%,海藻糖5%,PEG4000 0.25%)。结论在稳定剂作用下,酶联免疫吸附试验试剂盒可在4℃条件稳定放置1年。
Objective To develop ELISA kit stabilizer for each component ,with which the shelf life of kit could meet the com-mercial need .Methods A series of stabilizers for microplate ,enzyme-labeled antibody and standards were prepared by the means of experience and orthogonal approach ,through controlled trials ,screening out the best stabilizer formulations .Results A kind of coa-ted stabilizer （mass fraction ：bovine serum albumin 0 .5% ,gelatin 0 .25% ,trehalose 5% ,PEG4000 0 .1% ） and a kind of enzyme la-beled antibody stabilizer （mass fraction ：Bovine serum albumin 1% ,peptone 1% ,sucrose 10% ,trehalose 5% ,PEG4000 0 .25% ） were screened out .Conclusion The ELISA kits can be stable at 4 ℃ for 1 year after treated with the obtained stabilizers .
出处
《国际检验医学杂志》
CAS
2014年第18期2527-2528,共2页
International Journal of Laboratory Medicine
关键词
酶联免疫试剂盒
酶标板
酶标抗体
稳定剂
enzyme-linked immunosorbent assay kit coating plate enzyme labeled antibody stabilizer
