摘要
目的:观察抗郁散对慢性不可预见性轻度应激抑郁症(CUMS)大鼠的行为学变化与大脑皮质5-羟色胺2A受体(5-HT2AR)基因表达水平的变化,探讨抗郁散治疗抑郁症的机制。方法:将70只成年雄性SD大鼠,随机分为正常对照组、模型对照组、米氮平组、抗郁散高、中、低剂量组6组,利用CUMS方法复制抑郁模型。运用荧光Real Time PCR技术进行扩增,检测大鼠皮质5-HT2AR mRNA含量。结果:造模后,与正常对照组比较,造模组大鼠体质量增长缓慢(P<0.05)、水平运动减少、垂直次数得分下降及大鼠糖水消耗明显下降(P<0.01)。给药治疗后,与模型对照组比较,米氮平组、抗郁散高剂量组大鼠体质量增加(P<0.05),米氮平组、抗郁散高、中剂量组敞箱运动和糖水消耗显著增加(P<0.01);与正常对照组相比,模型对照组大鼠皮质5-HT2AR mRNA相对值含量明显增加(P<0.01);与模型对照组比较,抗郁散高、中剂量组和米氮平组大鼠皮质5-HT2AR mRNA相对值含量显著减少(P<0.01)。结论:抗郁散具有改善CUMS大鼠行为学变化的特征,能够下调大脑皮质5-HT2AR活性,再平衡中枢5-HT1AR与5-HT2AR的功能,是抗郁散的药效靶点之一。
Objective： Observation of the effects of Kangyusan on the CUMS rat behaviors and the 5-HT2 AR mRNA expression in the cerebral cortex of CUMS rat to explore its antidepressant mechanism. Methods： 70 adult male SD rats were randomly divided into normal control group, model control group, Mirtazapine group, high, medium and low dosage Kangyusan group. The depression rat model was established with the CUMS method. Fluorescence Real Time PCR technology was used to detect the 5-HT2 AR mRNA expression in the cerebral cortex of CUMS rat. Results： After model establishment, the growth of body weight, scores of horizontal motion and vertical motion and sucrose consumption in 6 model control groups was significantly lower than that in the normal control group（P〈0.05, P〈0.01）. After antidepressant treatment, the growth of body weight, the scores of horizontal motion and vertical motion and sucrose consumption in Mirtazapine group, high and medium dosage Kangyusan group were significantly higher than that in the normal control group（P〈0.01）; The 5-HT2 AR mRNA expression level in the cerebral cortex of CUMS rat in the model control group was significantly higher than that of normal control group（P〈0.01）; The 5-HT2 AR mRNA expression level in the cerebral cortex of CUMS rat in the normal control group, high, medium dosage Kangyusan group and Mirtazapine group were all significantly higher than that of model control group（P〈0.01）. Conclusion： Kangyusan can improve the behaviors of CUMS rats, reduce the activity of 5-HT2 AR in cerebral cortex, which may be one of the drug targets.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第10期3103-3106,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
贵州省自然科学技术基金项目(No.黔科合J字[2011]2302号)~~
