摘要
目的研究过敏性紫癜（HSP）患儿急性期外周血单核细胞（PBMC）TLR2,4,6的表达及其与血浆白细胞介素（IL）-17的相关性,探讨TLR2,4,6在HSP发病机制中的作用。方法选取2013年4月至12月青岛大学附属医院收治入院的HSP急性期患儿42例为研究对象（纳入HSP组）,年龄为4-13岁,男性患儿为25例,女性为17例。另选取同期于本院儿童保健科进行健康体检的30例儿童纳入对照组。采用流式细胞术检测HSP患儿PBMC TLR2,4,6蛋白表达情况,采用实时荧光定量PCR（RTPCR）法检测HSP患儿PBMC髓样分化蛋白88（MyD88）mRNA的相对表达水平,采用酶联免疫吸附测定（ELISA）法检测血浆白细胞介素（IL）-17水平。组间比较采用独立样本t检验,相关性分析采用Pearson相关检验。本研究遵循的程序符合青岛大学附属医院人体试验委员会所制定的伦理学标准,得到该委员会批准,并征得受试对象监护人的知情同意,并与之签署临床研究知情同意书。结果 1HSP患儿PBMC TLR2,4,6蛋白表达均显著高于对照组,差异均有统计学意义（t=9.432,P〈0.01;t=8.876,P〈0.01;t=10.486,P〈0.01）。2HSP组MyD88mRNA相对表达水平（1.279±0.419）较对照组水平（0.995±0.251）显著升高,二者比较,差异有统计学意义（t=2.463,P〈0.05）。3HSP组患儿血浆IL-17水平较对照组显著增高[（13.168±2.841）pg/mL vs（11.091±1.768）pg/mL],差异有统计学意义（t=2.641,P〈0.05）。4HSP组PBMC TLR2,4,6蛋白表达均与MyD88mRNA的相对表达水平呈显著正相关关系（r=0.850,P〈0.01;r=0.669,P〈0.01;r=0.788,P〈0.01）;与血浆IL-17水平均呈正相关关系（r=0.438,P〈0.01;r=0.404,P〈0.05;r=0.357,P〈0.05）。结论 TLR2,4,6信号途径的异常活化可能参与HSP发生发展,活化的TLR2,4,6可能通过上调辅助性T细胞（Th17）介导HSP的免疫发病机制。
Objective To study the expression of TLR2,4,6 protein in peripheral blood mononuclear cell（PBMC）and the relationship with helper T cell（Th）17 of children with Henoch-Schonlein purpura（HSP）,and to investigate the roles of the TLR2,4,6 in the pathogenesis of HSP.Methods Forty-two children with acute phrase HSP who were hospitalized from April 2013 to December 2013 in the Affiliated Hospital of Qingdao University were enrolled（HSP group）,aged 4-13.Among them,there were 25 males and 17 females.At the same time,another 30 healthy children from Child Health Division of the same hospital were served as control group.TLR2,4,6protein expression levels were detected by flow cytometric analysis.MyD88 mRNA expression levels in PBMC were detected by real-time fluorescent polymerase chain reaction（RT-PCR）.The levels of plasma interleukin（IL）-17 were detected by enzyme linked immunosorbent assay（ELISA）.In this study,t test and Pearson correlation test of statistical analysis were used between two groups.The study protocol was approved by the Ethical Review Board of Investigation of Affiliated Hospital of Qingdao University.Informed consent was obtained from all participates′parents.Results 1 Compared with control group,the expression level of TLR2,4,6 protein were remarkably increased in HSP group（t=9.432,P〈0.01;t=8.876,P〈0.01;t=10.486,P〈0.01）.2 The level of MyD88 mRNA in HSP group（1.279±0.419）was higher than that of control group（0.995±0.251）with significant difference（t=2.463,P〈0.05）.3The levels of plasma IL-17 of HSP group was significantly higher than that of control group[（13.168±2.841）pg/mL vs（11.091±1.768）pg/mL]with significant difference（t=2.641,P〈0.05）.4 The TLR2,4,6protein expression of PBMC and the level of MyD88 mRNA expression of HSP group showed a significant positive correlation（r=0.85,P〈0.01;r=0.669,P〈0.01;r=0.788,P〈0.01）;and its also had positive correlation with the level of plasma IL-17.Conclusions The aberrant activation of the
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2014年第5期55-59,共5页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
山东省优秀中青年科学家科研奖励基金(BS2011SW006)~~
