摘要
目的：观察血栓心脉宁对β-淀粉样蛋白（Aβ）致老年性痴呆（AD）大鼠行为学及大脑皮层和海马组织结构的影响，探讨血栓心脉宁对大鼠 AD的治疗作用。方法：选取100只雄性 Wistar大鼠，按体质量将大鼠随机分为假手术组、模型组、阳性药组（盐酸多奈哌齐，1.75 mg·kg-1）和血栓心脉宁1.1、2.2 g·kg-1剂量组。连续灌胃给药15 d。大鼠海马定位注射 Aβ建立大鼠 AD 模型。进行 Morris 水迷宫试验、避暗及病理学实验。结果：水迷宫试验，与模型组比较，血栓心脉宁1.1 g·kg-1剂量组大鼠第2、4和5天到达平台的潜伏期和游程明显缩短（P＜0.05或 P＜0.01）；血栓心脉宁2.2 g·kg-1剂量组大鼠第3～6天到达平台的潜伏期缩短（P＜0.05或P＜0.01），第3～5天到达平台的游程明显缩短（P＜0.05或P＜0.01）；第7天血栓心脉宁各剂量组大鼠在90 s 内经过平台的次数、平台停留时间、平台区停留距离、有效区停留时间、有效区停留距离、平台停留时间/总时间、平台停留距离/总路程和有效区停留时间/总时间均明显增加（P＜0.05或 P＜0.01）。避暗实验中，与模型组比较，血栓心脉宁各剂量组大鼠第2天的错误潜伏期及错误次数无显著差异（P＞0.05）。病理学观察，血栓心脉宁各剂量组大鼠与模型组比较其病理学表现无明显差异，大脑皮层神经细胞有变性、坏死；海马神经细胞数少，层次不清楚，可见变性、坏死细胞。结论：血栓心脉宁可改善 AD大鼠的学习记忆功能，但对其病理学病变无明显改善作用。
Objective To observe the influence of XueShuanXinMaiNing（XSXMN）in the behavior and structures of cerebral cortex and hippocampus of the rats withβamyloid protein（Aβ）-induced Alzheimer’s disease（AD）,and to explore its therapeutic effects on the rat AD.Methods 100 male Wistar rats were selected.According to weight, the rats were randomly divided into sham operation group, model group, positive drug group （donepezil hydrochloride,1.75 mg· kg-1 ）,XSXMN 1.1 g· kg-1 group and XSXMN 2.2 g· kg-1 group. The rat AD models were made by injecting Aβinto hippocampus.After oral administration for 15 d,Morris water maze test, dark avoidance task and pathology test were performed.Results In Morris water maze test,compared with model group,the latency and swimming distance to platform of the rats in XSXMN 1.1 g·kg-1 group were decreased on the 2nd,4th and 5th day（P〈0.05 or P〈0.01）;in XSXMN 2.2 g·kg-1 group,the latency to platform of the rats were decreased from the 3nd to 6th day（P〈0.05 or P〈0.01）,the swimming distances to platform of the rats were decreased from the 3rd to 5th day（P〈0.05 or P〈0.01）.On the 7th day,in XSXMN groups,the times of passing platform,time of staying on platform,distance of staying on platform,time of staying in effective area, distance of staying in effective area, time of staying on platform/total time, distance of staying on platform/total distance,time of staying on platform/total time were all increased significantly（P〈0.05 or P〈0.01）within 90 s. In dark avoidance task,compared with model group,the error latency and the error times of the rats in XSXMN groups had no obvious change on the 2nd day.The pathological results showed that there were degeneration nerve cells and necrosis nerve cells in the rat cerebral cortex in XSXMN groups,while in the rat hippocampus there were less number of nerve cells with obscure cell layer and many degeneration and necrosis cells were found;compared with model group,there was no obvious improvement.Conclusion
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2014年第5期985-990,I0004,共7页
Journal of Jilin University：Medicine Edition
基金
吉林省中医药管理局科研基金资助课题(2010-115)
