摘要
研究了新型环烷基磺酰胺类化合物N-(2,4,5-三氯苯基)-2-氧代环己烷基磺酰胺(简称化合物108)对灰葡萄孢菌丝生长、孢子形成和萌发以及菌核产生等不同生育阶段的抑制作用及其对菌丝致病力和形态结构的影响。结果表明:化合物108对灰葡萄孢菌丝生长和孢子形成及孢子萌发具有明显的抑制作用,其EC50值分别为6.90、4.70和4.11μg/mL;菌核形成受到明显抑制,当药剂质量浓度达20μg/mL时,无菌核产生。经化合物108处理后的灰葡萄孢菌丝致病力下降,40μg/mL处理的菌丝致病力显著低于对照。超微结构观察结果表明,化合物108能导致灰葡萄孢菌丝萎缩、塌陷和变形,菌体细胞壁增厚、皱缩及分层。
The antifungal activity of cycloalkyl sulfonylureas N-（2,4,5-trichlorophenyl）-2-oxocyclohexylsulfonamide （ compound 108 for short） against Botrytis cinerea was investigated in vitro. The results showed that compound 108 could inhibit different stages of B. cinerea including hyphal growth, spore production and germination, with EC50 values of 6. 90, 4. 70 and 4. 11 μg/mL, respectively. The sclerotic production and pathogenicity of B. cinerea could also be inhibited by compound 108, and the hyphal could not produce sclerotia under 20 μg/mL of the compound. The pathogenicity of B. cinerea mycelia treated by compound 108 at 40 μg/mL was significantly lower than that of the control. Morphological and ultrastructural studies showed that compound 108 could cause hyphae collapse, deforming, and cell wall shrinkage, thickening and layering of B. cinerea.
出处
《农药学学报》
CAS
CSCD
北大核心
2014年第5期523-528,共6页
Chinese Journal of Pesticide Science
基金
公益性行业(农业)科研专项经费资助(201303025)
国家自然科学基金项目(31101466)
