摘要
目的建立同时测定雪松松针中没食子酸、原儿茶酸和儿茶素含量的高效液相色谱法。方法采用TC-C18（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇（A）-0.2%磷酸水溶液（B）梯度洗脱（0~8 min,10%A,8~45 min,10%→30%A）,流速：1.0 mL·min-1,检测波长：280 nm,柱温：25℃。结果雪松松针中没食子酸、原儿茶酸和儿茶素依次在0.4~4.0,4~40和2~20μg·mL-1内呈良好线性关系,相关系数r分别为0.999 8,0.999 9,0.999 9,加样回收率（n=9）分别为104.7%,99.5%和105.8%。结论该方法适用于雪松松针中没食子酸、原儿茶酸和儿茶素的含量测定,方法简单、快速、高效。
OBJECTIVE To develop an HPLC method for simultaneous determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara. METHODS Agilent TC-C18 column （250 mm×4.6 mm,5μm） was adopted. The mobile phase was methanol （A）-0.2% phosphoric acid （B） with gradient elution （0-8 min, 10%A; 8-45 min, 10%→30%A） at the flow rate of 1.0 mL.min-1. The column temperature was 25 ℃ and the detection wavelength was 280 nm. RESULTS Calibration curves were found to be linear in the ranges of 0.4-4.0 μg.mL-1 for gallic acid, 4-40 μg.mL-1 for protocatechuic acid and 2-20 μg.mL-1 for catechin. The correlation coefficients were 0.999 8, 0.999 9, 0.999 9. The average recoveries（n=9） of gallic acid, protocatechuic acid and catechin were 104.7%, 99.5% and 105.8%. CONCLUSION The method is simple, rapid and efficient, which is suitable for the determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara.
出处
《中国现代应用药学》
CAS
CSCD
2014年第9期1104-1107,共4页
Chinese Journal of Modern Applied Pharmacy
基金
甘肃省科技支撑计划项目(1204FKCA152)
甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(zzy-2011-05)
甘肃省卫生厅中医药科研项目(GZK-2012-29)
关键词
雪松松针
没食子酸
原儿茶酸
儿茶素
含量测定
高效液相色谱法
pine needles of Cedrus deodara
gallic acid
protocatechuic acid
catechin
contents determination
HPLC
