摘要
The Chinese yew（Taxus wallichiana）,which is widely distributed in the Himalayas and in southern China,is now on the edge of extinction.In order to understand the evolutionary processes that control the current diversity within this species at the genetic and ecological levels,its genetic patterns and range dynamics must first be identified and mapped.This knowledge can then be applied in the development of an effective conservation strategy.Based on molecular data obtained from 48 populations of T.wallichiana,we used GIS-based interpolation approach for the explicit visualization of patterns of genetic divergence and diversity,and a number of potential evolutionary hotspots have been specifically identified within the genetic landscape maps.Within the maps of genetic divergence and diversity,five areas of high inter-population genetic divergence and six areas of high intra-population genetic diversity have been highlighted in a number of separate mountain regions,and these evolutionary hotspots should have the priority to be protected.Furthermore,four geographical barriers have been identified： the eastern Himalayas,the Yunnan Plateau,the Hengduan Mountains and the Taiwan Strait.According to ecological niche modeling（ENM）,the populations of T.wallichiana within the Sino-Himalayan Forest floristic subkingdom experienced westward expansion from the periods of Last Inter-glacial to Last Glacial Maximum（LGM）.Following the LGM,the distribution range overall became reduced and fragmented.These findings challenge the classic mode of contraction-expansion in response to the last glaciation.In conclusion,our findings suggest that the changes in geographical landscapes and climate that occurred during the Quaternary resulted in current genetic landscape patterns.
广泛分布于喜马拉雅山脉和我国华南地区的西藏红豆杉频临灭绝,揭示空间遗传结构和物种分布变化对于理解西藏红豆杉的进化过程及其物种保护尤为重要。基于西藏红豆杉48个种群的分子变异数据,利用反距离权重空间差值方法得到遗传多样性和遗传分化分布图,识别出6个遗传多样性和5个遗传分化高值区域,这些重点区域主要位于我国南方的几个山区,这些区域在未来应给予重点保护;基于Monmonier算法识别出4条地理隔离:东喜马拉雅山脉、横断山脉、云南高原和台湾海峡;利用生态位模型模拟三个历史时期的物种分布格局,从末次间冰期至末次冰盛期,物种经历一次向高原西部的面积扩张,这与典型的冰期物种退缩模式不同;末次冰盛期之后,物种面积逐渐缩小,呈现破碎化分布。因此,地理景观特征和第四纪气候波动对西藏红豆杉遗传结构和分布格局影响很大。
基金
National Basic Research Program of China(No.2010CB951704)
National Natural Science Foundation of China(No.41271068)
