摘要
目的:研究环维黄杨星D对高交感活性诱导大鼠实验性心肌损伤氧化应激及能量代谢障碍的改善作用。方法:SD大鼠随机分为正常对照组、模型组、维生素E组及环维黄杨星D低、高剂量组,除正常对照组外其余各组均腹腔注射去甲肾上腺素(Noradrenaline,NE)复制实验性心肌损伤模型,同时灌胃给予相应药物,连续给药16d,期间检测各组动物体质量变化,16 d后进行心指数、羟脯氨酸含量、组织病理学检查、氧化/抗氧化指标(MDA、SOD、CAT、GSH-Px、T-AOC)、能量代谢指标(Na+,K+-ATPase、Ca2+,Mg2+-ATPase)的测定。结果:环维黄杨星D低、高剂量组均不同程度增加动物体质量,降低心指数及羟脯氨酸含量,改善氧化/抗氧化失衡,改善能量代谢障碍。结论:环维黄杨星D对高交感活性诱导的实验性心肌损伤氧化应激及能量代谢障碍具有显著的保护作用。
Objective: To investigate the ameliorated effect of CVB-D on oxidative stress and energy metabolism in experimental cardiac injuried rats induced by sympathetic overactivity in vivo.Methods: SD rats were randomly divided into five groups as following:control group,model group,Vitamin E 150 mg /kg group,CVB-D low dose and high dose groups,respectively.The rat experimental cardiac injury model was established by exposed to norepinephrine( NE) 3 mg /kg by ip for 16 d.The drugs were administrated to rat for 16 d by ig.The body weight of rats were monitored during all of the experimental period.At the designing ending-time point the indexes were assayed as following: cardiac index,hydroxyproline,histopathologically examination,oxidative stress( MDA,SOD,CAT,GSH-Px and T-AOC) and energy metabolism indicatricle( Na+,K+-ATPase,and Ca2 +,Mg2 +-ATPase).Results: After exposed with NE for 16 d,the rats of model group was appeared dysfunction of oxidative stress and energy metabolism such as decreasing body weight,increasing cardiac index and hydroxyproline in cardiac tissue,decreasing Na+,K+-ATPase and Ca2 +,Mg2 +-ATPase activities,and deteriorating the oxidative stress.Treated with CVB-D could ameliorate all of the exacerbated indexes.Conclusion: CVB-D has protective effect against oxidative stress and energy metabolism in rats of experimental myocardial injury induced by sympathetic overactivity.
出处
《中药材》
CAS
CSCD
北大核心
2014年第7期1213-1217,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81173586)
教育部新世纪优秀人才支持计划(NCET-13-0747)
贵州省省长专项资金项目(黔科教办[2011]28号)
贵州省社会发展攻关项目(黔科合SY字[2011]3011号
[2012]3085号)