摘要
目的:探讨参地补肾胶囊对肾小球硬化大鼠BMP-7、CTGF表达的影响。方法:采用单侧肾脏切除同时尾静脉注射阿霉素的方法建立肾小球硬化大鼠模型,实验分为正常对照组、模型组、西药(洛汀新)对照组、中药(苏黄泄浊丸)组和参地补肾胶囊组,分组给药处理8周。检测各组大鼠24小时尿蛋白定量、血肌酐(SCr)、尿素氮(BUN)、血浆蛋白(ALB);光镜观察大鼠肾脏病理学变化;免疫组化法检测BMP-7、CTGF表达。结果:各治疗组大鼠24小时尿蛋白及血SCr、BUN、ALB较模型组明显改善(P<0.05)。各给药组大鼠肾脏病理变化明显改善。各治疗组均能抑制CTGF的表达(P<0.05),上调BMP-7表达(P<0.05);参地补肾胶囊组作用明显优于两阳性对照组(P<0.05)。结论:参地补肾胶囊对肾小球硬化大鼠肾功能具有保护作用,上调BMP-7表达、抑制CTGF表达可能是其部分作用机制。
Objective：To explore the effects of Shendi bushen capsule on BMP-7、CTGF expressions in rats with glomerular sclerosis.Methods：Unilateral nephrectomy and injection of adriamycin by tail vein to establish the model of glomerular sclerosis.SD rats were randomly divided into normal control group , model group, western medi-cine （lotensin） group,Chinese medicine （Suhuang xiezhuo pill） group and Shendi bushen capsule group,each group was given the corresponding medicine for 8 weeks.rats’24hours urinary protein,serum creatinine（SCr）、urea nitrogen （BUN）、plasma albumin（ALB）were determined;the pathological changes of rats’kidney were observed by light micro-scope;the expressions of BMP-7、CTGF in renal tissue was determined by immunohistochemical method.Results：To compare with model group,24 hours urinary protein and blood SCr、BUN and ALB were improved significantly in each treatment group（P〈0.05）.the pathologic structure changes of renal glomerular, tubules, interstitial and vascular were improved obviously in each treatment group.The expression of CTGF was inhibit and expression of BMP-7 was up-regulated in each treatment group, the effects of Shendi bushen capsule group were the most obvious（P〈0.05）.Conclusion：Shendi bushen capsule can protect rats’ renal function ,its mechanism may be related to increasing the expression of BMP-7 and inhibiting CTGF expression.
出处
《中国中医药科技》
CAS
2014年第5期501-503,共3页
Chinese Journal of Traditional Medical Science and Technology
