摘要
目的观察蒙药地格达-4对酒精性脂肪肝模型大鼠肝组织及血清应激氧化指标的影响。方法选取Wistar大鼠进行造模,然后随机分为空白组、模型组、硫普罗宁组和地格达-4高、中、低剂量组。空白组和模型组以羧甲基纤维素钠,硫普罗宁组以硫普罗宁40 mg/kg,地格达-4高、中、低剂量组分别以1400、700、350 mg/kg地格达-4灌胃4周后,处死,腹主动脉采血,取肝脏,检测超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-px)等指标。结果地格达-4高、中剂量组与模型组间各项指标比较均有显著差异(P<0.01);而地格达-4低剂量组与模型组间只有血清MDA有显著差异(P<0.01),其他指标均无明显差异;地格达-4中剂量组与地格达-4高剂量组间肝组织和血清SOD、MDA、GSH-px比较有显著差异(P<0.01)。结论地格达-4能显著升高酒精性脂肪肝模型大鼠血清和肝组织当中的SOD和CSH-px,同时能显著降低MDA的含量。
Objective： To observe the influence of Mongolian medicine Digeda-4 on the liver tissue and serum oxidative stress indicators in rats with alcoholic fatty liver. Methods： After establishment of model,wistar rats were randomly divided into blank group, model group,tiopronin group, Digeda-4 low, and medium and high dose groups. Blank and model groups were given carboxymethyl cellulose natrium;Tiopronin group were given Tio- pronin 40 mg/kg;Digeda-4 low,middle and high dose groups were given 1400 mg/kg,700 mg/kg,350 mg/kg of Digeda-4 respectively. After 4 weeks, all rats were put to death and blood samples were collected from abdominal aorta. The liver was taken and indicators such as superoxide,dismutase,malonaldehyde,and glutathione peroxi- dase were tested. Results： All the indicators in Digeda-4 high,middle dosage groups and model group had sig- nificant differences （P〈 0.01）. Only serum MDA had significant difference between Digeda-4 low dosage group and model group（P〈 0.01 ）, other indicators had no difference;SOD, MDA and GSH-px had significant differences between Digeda-4 middle dosage group and Digeda-4 high dosage group （P〈 0.01 ）. Conclusion： Digeda-4 can significantly improve SOD and GSH-px in liver tissue and serum oxidative stress indicators of rats with alcoholic fatty liver, and significantly reduce the content of MDA.
出处
《中国中医急症》
2014年第9期1589-1590,1613,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81160549)
关键词
蒙药
地格达-4
酒精性脂肪肝
大鼠
氧化指标
Mongolian medicine
Digeda-4
Alcoholic fatty liver
Rats
Oxidation indices
