摘要
目的:研究蒙药地格达-4对四氯化碳(CCl4)致大鼠肝损伤的保护作用。方法:SD大鼠按3mL·kg-1腹部皮下注射40%CCl4橄榄油溶液,每周2次,持续1月复制肝损伤模型。实验分为6组,即空白对照、模型、联苯双酯(0.075g·kg-1)和地格达-4高、中、低剂量(1.5、0.75、0.38g·kg-1)组。给药40d后测定血清中天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰转移酶(GGT)的活性,总胆红素(TBIL)的含量,肝脏指数及肝组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、人谷胱甘肽过氧化物酶(GSH-Px)的活性,丙二醛(MDA)的含量,总抗氧化能力(T-AOC);并对肝组织做病理学检测。结果:地格达-4能显著降低血清中AST、ALT活性及肝脏指数(P<0.05),降低TBIL、ALP、GGT含量;升高肝组织中SOD活性,降低MDA含量和CAT、GSH-PX、T-AOC活性;减轻肝组织变性、坏死等病理改变。结论:地格达-4对CCl4所致大鼠肝损伤具有保肝作用,作用机制可能与其抗脂质过氧化作用相关。
OBJECTIVE：To study the protection effect of mongolian medicine Digeda-4 against hepatic injury induced by CC14 in rats.METHODS：SD rats were injected in abdominal wall with 40% CCl4（3 mL·kg^-1）twice a week for one month to induce hepatic injury model.Model rats were divided into blank group,model group,bifendate group（0.075 g·kg^-1）,Digeda-4 low dosage,middle dosage and high dosage groups（1.5,0.75,0.38 g·kg^-1）.After 40 days of treatment,the activity of AST,ALT,ALP,GGT and the content of TBIL in serum were determined as well as hepatic index.The activity of SOD,CAT,GSH-Px and content of MDA in hepatic tissue were detected as well as T-AOC.Pathological test of liver was conducted.RESULTS：Digeda-4 could decrease the activity of AST and ALT,hepatic index and content of TBIL,ALP,GGT in serum.It also increased the activity of SOD in hepatic tissue and reduced the content of MDA,CAT,GSH-Px,T-AOC and necrosis of liver tissues.CONCLUSION：Digeda-4 has protection effect on hepatic injury in rats and the mechanism is related to anti-lipid peroxidation possibly.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第19期1729-1731,共3页
China Pharmacy
基金
国家自然科学基金面上项目(30873451)
关键词
蒙药
地格达-4
四氯化碳
肝损伤
抗脂质过氧化
Mongolian medicine
Digeda-4
CCl4
Hepatic injury
Anti-lipid peroxidation
