摘要
目的:探讨枸杞多糖对H2O2诱导胰岛INS-1细胞凋亡和胰岛素分泌功能的影响。方法:采用H2O2诱导INS-1细胞凋亡模型,用100 mg/L枸杞多糖处理INS-1细胞,MTT法检测细胞活力,放射免疫法检测胰岛素含量,流式细胞仪检测细胞凋亡,荧光定量PCR检测Bcl-2、Bax和Caspase-3的mRNA表达。结果 100 mg/L枸杞多糖能明显提高H2O2处理INS-1细胞的活力(P<0.01),促进细胞基础和葡萄糖刺激的胰岛素分泌(P<0.05,P<0.01),减少细胞凋亡(P<0.01),同时Bcl-2的表达明显升高(P<0.01),Bax和Caspase-3的表达明显降低(P<0.01,P<0.01)。结论:枸杞多糖可能通过促进Bcl-2的表达,抑制Bax和Caspase-3的表达,以降低H2O2诱导的INS-1凋亡,同时促进INS-1细胞的胰岛素分泌。
Objective： To investigate the effects of Lycium barbarum polysaccharide （LBP） on the apoptosis and insulin secretion of INS-1 cell treated with H2O2 Method：The cell apoptosis model of INS-1 was induced by H202 , and then the cells were treated with LBP （ 100 mg/L）. The viability and insulin secretion of INS-1 were measured by MTT and immunoradiometric assay, respectively. Cell apoptosis was determined with flow cytometer, and the mRNA expression levels of Bcl-2,Bax and Caspase-3 were detected by qRT-PCR. Results： 100 mg/L LBP could significantly improve H202 treated INS-1 cell viability （P 〈 0. O1 ）, increase basal and glucose stimulated insulin secretion （P 〈 0. 05, P 〈 O. O1 ）, and reduce cell apoptosis（P 〈 0.01 ）. Simultaneously, the mRNA expression level of Bcl-2 was significantly upexpressed （P 〈 0. 01 ）, Bax and Caspase-3 were significantly downexpressed （P 〈 0.01 ）. Conclusion： LBP may improve insulin secretion of INS-1 cell treated with H202 by inhibiting cell apoptosis through promote Bcl-2 expression and reduce Bax and Caspase-3 expression.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2014年第9期1215-1217,共3页
JOURNAL OF BASIC CHINESE MEDICINE
基金
河北省教育厅科技-青年基金项目(QN20131039)
关键词
枸杞多糖
INS-1
细胞凋亡
胰岛素分泌
Lycium barbarum polysaccharide
INS-1 cell
apoptosis
insulin secretion
