摘要
比较菲牛蛭、日本医蛭、宽体金线蛭和八目石蛭盐浸提物的抗凝活性,分别为(106.39±48.57)U/g、(29.44±11.31)U/g、(8.06±0.48)U/g和(0.00±0.00)U/g,其中菲牛蛭与日本医蛭、八目石蛭和宽体金线蛭差异极显著(P<0.01)。冻存0、2、16、24、34个月的菲牛蛭抗凝活性依次为(134.72±51.11)U/g、(117.33±16.04)U/g、(76.16±20.60)U/g、(41.94±4.11)U/g和(21.39±0.96)U/g,随冻存时间延长而下降。
The anti-thrombin activity was studied in four species of leeches Poecilobdella manillensis,Hirudo nipponia,Erpobdella octoculata and Whitmania pigra,and cryopreservation（-20℃）on anticoagulant activity was investigated in leech P.manillensis by anti-thrombin bioactivity method at（24±2）℃.The results showed that anti-thrombin bioactivity of four species of leeches were in the following decreasing order：P.manillensis,H.nipponia,W.pigraand E.octoculata,and the anti-thrombin bioactivity were（106.39±48.57）U/g,（29.44±11.31）U/g,（8.06±0.48）U/g and（0.00±0.00）U/g,respectively.Extracts of P.manillensis had higher anti-thrombin activity than that of other species of leeches（P〈0.01）.Anticoagulant activities of P.manillensis preserved in-20℃ were（134.72±51.11）U/g for 0month,（117.33±16.04）U/g,for 2months,（76.16±20.60）U/g for 16 months,（41.94±4.11）U/g for 24 months and（21.39±0.96）U/g for 34 months.The anticoagulant activity of P.manillensis was negatively correlated to months of cryopreservation.
出处
《水产科学》
CAS
北大核心
2014年第9期591-593,共3页
Fisheries Science
基金
辽宁省海洋与渔业厅项目(200903)
关键词
水蛭
冻存
抗凝活性
leech
cryopreservation
anticoagulant activity
