摘要
以秦巴山区黑木耳主要栽培菌株为材料,采用不同紫外照射时间和温度梯度筛选高温黑木耳品种。结果表明,黑木耳原生质体紫外诱变的最佳时间为120-180 s,再生菌落的致死率为64%-82%,通过40℃高温初步筛选得到适合秦巴山区栽培的黑木耳菌株YBZ4。
With the Auricularia auricula strains cultivated in Qinling-Bashan Mountain Areas,excellent strains are expected to be found out by ultraviolet mutagenesis method.Different ultraviolet radiating time and temperature gradients are adopted to select the excellent high temperature resistant Auricularia auricula strains.The best time for ultraviolet mutagenesis is 120 - 180 s,and the preliminary Auricularia auricula strains suitable to Qinling-Bashan Mountain Areas can be sorted out from the whole samples.The high temperature resistant Auricularia auricula strain which is suitable to Qinling-Bashan Mountain Areas is YBZ4.
出处
《陕西理工学院学报（自然科学版）》
2014年第5期46-50,共5页
Journal of Shananxi University of Technology:Natural Science Edition
基金
陕西省科学技术研究发展计划项目(2014K01-16-03)
陕西省科技统筹创新工程计划项目(2012HBGC-20)
陕西理工学院研究生创新基金资助项目(SLGYCX1309
SLGYCX1418)
关键词
黑木耳
原生质体
紫外诱变
筛选
高温
Auricularia auricula
protoplast
ultraviolet mutagenesis
choose
high temperature
