摘要
目的:对二甲基亚硝胺(DMN)及硫代乙酰胺(TAA)诱导的肝纤维化进行形态学比较。方法:0.5%DMN腹腔注射(2mL/kg,每周3次,连续4周)或自由饮用TAA水溶液(0.03%TAA饮用9周后改用0.04%TAA饮用6周)两种方法制备大鼠肝纤维化模型,在造模完成后,恢复4周,比较两种造模方法成功率、肝内胶原沉着情况及α-肌动蛋白(α-SMA)表达情况。结果:DMN造模动物死亡率高(>30%),TAA模型无动物死亡;DMN模型纤维化严重程度较一致,造模成功率均较TAA模型高(P<0.01);DMN模型胶原沉着面积与TAA模型相当;DMN模型α-SMA表达高于TAA模型(P<0.01)。结论:腹腔注射DMN法诱导肝纤维化优于自由摄取TAA法。
AIM： To compare the morphologi- cal differences of liver between the two liver fi- brosis models induced by dimethylnitrosamine （DMN） and thioacetamide（TAA）. METHODS： 0.5% DMN （2 mL/kg; 3 times/week） was in- jected into the abdominal cavity of SD rats for 4 weeks to create one kind of liver fibrosis model. To create another kind of liver fibrosis models, 0.03% TAA in drinking water was administered orally to SD rats for 9 weeks and then 0.04% TAA was administered orally to SD rats for oth- er 6 weeks. After being treated with DMN or TAA, animals were not treated for 4 weeks for recovery. The morphology of liver was evaluated in two models based on the H＆E stain and the severity of liver fibrosis was compared in two models with collagen volume fraction and the protein expression of α-SMA. RESULTS： Themortality of rat in the model induced by DMN was high（30%） but no animal was dead in the model induced by TAA. The success rate in the model induced by DMN were higher than those induced by TAA （P〈0.01）. And the uniformi- ty of liver fibrosis severity in the model induced by DMN is better than the latter. The two mod- els had the similar collagen volume fractions. The protein expression of α-SMA in the model induced by DMN was higher than that induced by TAA （P〈0.01）. CONCLUSION. The model induced by DMN is better than that induced by TAA.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2014年第7期747-751,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
二甲基亚硝胺
硫代乙酰胺
肝纤维化
形态学
dimethylnitrosamine
thioacet-amide
liver fibrosis
morphological difference
