摘要
以闽花6号为材料,利用SMART技术成功构建了花生根全长cDNA文库.原始文库滴度为1.3×10^6cfu/mL,重组率达95.8%,插入片段大小为750～2000bp,符合全长cDNA文库的质量标准.随机挑选35个单克隆进行双向测序,共获得30条有效序列.通过与NCBI非冗余蛋白质数据库进行比对和注释,获得已知功能基因或具推测功能的基因15个,未知功能基因7个,新基因13个,其中34个（97.1%）基因为花生未报道的基因.因此,该文库的构建为克隆和研究花生根部重要表达基因、从分子水平上揭示花生根的生长发育规律提供了基础.
Root cDNA library of peanut was constructed with the roots of Arachis hypogaea cv Minhua 6 using SMART technique. The original library had a titer of 1. 3 × 106 cfu /mL,of which 95. 8% clones were recombinant and insert cDNAs were from 750 bp to 2 000 bp. 35 clones from the cDNA library were sequenced and 30 valid sequences were generated. 25 known or hypothentical functional genes,7 unidentified genes and 13 novel gene were annotated by Blastx searches against the NCBI non- redundant protein database. Among these genes,34 genes( 97.1%) had not been reported in peanut. The result revealed that expression genes in peanut root reported online at present were still rare. The full length cDNA library of peanut roots constructed in this study would be beneficial to mine important functional genes expressed in peanut roots and supply basis to study on the growth and development of peanut roots from molecular level.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2014年第5期653-660,共8页
Chinese Journal of Oil Crop Sciences
基金
科技部国际科技合作计划(2008DFA31450)
国家863计划(2013AA102602-5)
福建省科技厅高校产学合作科技重大项目(2011N51010064)
