摘要
目的调查不同群体树鼩弓形虫的感染情况,为建立树鼩寄生虫学监测指标提供依据。方法分别从野外来源、人工驯化一年和人工繁殖的子一代三个树鼩群体中,随机采集动物全血样本各40份,用间接血凝试验(IHA)检测弓形虫抗体,PCR方法检测其核酸,根据两种方法的检测结果分析树鼩弓形虫的感染情况。结果IHA和PCR检测结果显示,120份树鼩血样均为阴性,两种检测方法结果一致。结论本次调查三个群体来源的树鼩均未感染弓形虫,树鼩对弓形虫的易感情况还需要通过扩大样本量和感染性实验来进一步验证。
Objective To investigate the infection status of Toxoplasma gondii in different colonies of tree shrews and then provide the basis for parasitological monitoring .Methods Each of the forty blood samples were randomly collected from three tree shrews colonies ： wild origin, domesticated and first generation, respectively.Both indirect hemagglutination test （IHA） and PCR assay were used to detect the Toxoplasma gondii.Results No positive sample of Toxoplasma gondii was detected from either IHA or PCR results .The results from IHA and PCR assays were in coincidence with each other.Conclusions According to the survey none of the tree shrews from the three groups is infected with Toxoplasma gondii.More samples or infection experiments are needed to determine whether tree shrews can be infected with Toxoplasma gondii.
出处
《中国比较医学杂志》
CAS
2014年第8期28-30,46,共4页
Chinese Journal of Comparative Medicine
基金
国家科技支撑计划(2011BAI15B01-21
2012BAI39B01
2014BAI01B01)
云南省科技创新平台建设(2013DA002)
关键词
树鼩
弓形虫
间接血凝试验
聚合酶链反应
Tree Shrews
Toxoplasma gondii
Infection
Indirect hemagglutination Test
PCR
