摘要
目的研究不同浓度孕酮(PRO)暴露对雌性食蚊鱼(Gambusia affinis)的形态雄性化生物学效应。方法将性未成熟的雌性食蚊鱼个体分别静水暴露于0.5、5、50和500 nmol/L不同浓度的PRO实验组中,并设置对照组和平行组。持续暴露42 d后观察与测定食蚊鱼的体长、体重、臀鳍第3鳍条和第14,15,16椎肋骨形态雄性化变化等指标,同时观察卵巢发育状况。结果食蚊鱼暴露42 d后,与对照组相比,50和500 nmol/L实验组食蚊鱼的体长(BL)有极显著减少(分别P<0.01);无论是低浓度组还是高浓度组,暴露实验后体重(BW)都有显著性或极显著下降(P<0.05或P<0.01);PRO暴露对食蚊鱼身体健康指数(CF)的影响,只有在5 nmol/L浓度时差异有显著性(P<0.05);500 nmol/L浓度处理后,第3臀鳍条分节数(FJ)、长度(FL)和最宽处宽度(FW)差异有显著性(P<0.05),表明形态雄性化变化明显;其他浓度组则无明显区别(P>0.05);500 nmol/L PRO实验组中,雌性食蚊鱼的第14,15,16椎肋骨的L值、D值和L!D值都出现极显著性差异(分别P<0.01),表明形态雄性化变化明显;组织切片观察揭示,暴露组食蚊鱼卵巢中的卵母细胞发育受到不同程度的抑制,II、III时相卵母细胞胞核发生肿胀现象。结论孕激素暴露诱导雌性食蚊鱼形态雄性化的生物效应十分明显。
Objective The aim of this study was to investigate the masculinizing effects of progesterone （ PRO） exposure at different concentrations on the morphology of female mosquitofish （Gambusia affinis）.Methods Immature female G.affinis individuals were put into static water with 0.5, 5, 50 and 500 nmol/L progesterone （n=56）, respective-ly.In addition, control group and parallel groups were set up .After 42-day-long exposure, we measured four main indexes of the G.affinis：the body length, body weight, morphological changes in the anal fin 3rd fin and the 14, 15 and 16 verte-bral ribs.We also observed the state of ovarian development .Results The body lengths （BL） of experimental groups ex-posed at concentration of 50 and 500 nmol/L progesterone for 42 d showed significant differences , respectively （ P＆lt;0.01 for both）, when compared with those of the control group .The body weights （BW） of experimental groups exposed to pro-gesterone at all concentrations were significantly or very significantly decreased （P＆lt;0.05 or P＆lt;0.01）.Only the proges-terone at 5 nmol/L concentration showed significant effect on the body health index （CF） （P＆lt;0.05）.The section number （FJ） of the anal fin 3rd fin, the section length （FL）and the widest portion width （FW）in the experimental groups had sig-nificant difference （P＆lt;0.05）, respectively, showing significant changes in morphology after the exposure to 500 nmol/L progesterone .However , there was no significant difference between the values resulted by progesterone in other concentra -tions （P＆gt;0.05）.When exposed to 500 nmol/L PRO, the L, D and L：D values of the 14th, 15th and 16th vertebral ribs of the female G.affinis had very significant difference （ P＆lt;0.01 for all ） , respectively , indicating significant changes of morphological masculinization .Histological examination revealed that the developmental status of the ovaries of G.affinis in the experimental groups were to different degrees inhi
出处
《中国实验动物学报》
CAS
CSCD
2014年第4期31-36,I0002,I0003,共8页
Acta Laboratorium Animalis Scientia Sinica
基金
华南师范大学第8期大学生课外科研项目
广东高校城市水环境生态治理与修复工程技术研究中心建设项目(2012gczxA004)
