摘要
目的:通过观察面神经损伤后神经元型一氧化氮合酶(neuronal NOS,nNOS)在面神经核团中的表达,探讨NO在面神经损伤后的作用。方法:健康成年SD大白鼠20只,其中5只作为正常对照。其余15只为面神经损伤组,在双侧面神经下颊支制作挤压伤。术后1、2、3、4、5周各取3只损伤组大鼠作面神经核团nNOS表达的免疫组化观察。结果:正常情况下面神经元不表达nNOS。面神经损伤后,出现运动神经元变性,其神经元细胞nNOS免疫染色为阳性,并持续至损伤后第5周。结论:面神经损伤后其运动神经元的核团中会出现nNOS的表达。
Objective： To observe the variation of neuronal nitric oxide synthase （nNOS） expression in injured facial nerve tissue. Method： 20 adult male SD rats were used in this study, among them, 5 rats were normal controls. In experiment group, a compression injury was applied at one branch of bilateral facial nerve. Every 3 rats in experiment group were sacrified for nNOS immunostaining in a period of 1, 2, 3, 4 and 5 weeks respectively. Results： In normal facial nerve tissue, nNOS expression was not detected. In the injured facial nerve neurons, nNOS immunostaining expression was positive, and lasting up till the fifth weeks. Conclusion： After the nerve injury, the expressing of nNOS was observed in the facial nucleus, which may be related to the death of neuron.
出处
《口腔颌面外科杂志》
CAS
2014年第4期272-275,共4页
Journal of Oral and Maxillofacial Surgery
关键词
面神经
神经再生
神经元型一氧化氮合酶
鼠
facial never
nerve regeneration
neuronal nitric oxide synthase
rats
