摘要
目的:探讨脊神经根性撕脱伤后脊髓运动神经元中Eta1mRNA表达的意义.方法:首先建立脊神经根性撕脱伤的动物模型,采用原位杂交和免疫组织化学方法检测损伤前后Eta1mRNA在脊髓运动神经元中表达的变化.结果:损伤前Eta1在脊髓运动神经元中微量表达.损伤后3d,损伤侧脊髓运动神经元总数减少31.6%,但表达Eta1的运动神经元数量增加52.5%.结论:脊神经根撕脱后Eta1在脊髓运动神经元内上调表达可能是对神经元本身的一种保护.
AIM： To explore the alteration of Eta-I expression in motoneurons following spinal root avulsion and its significance. METHODS： Animal model of spinal root avulsion was established and immunohistochemistry and in situ hybridization were employed to investigate whether spinal root avulsion will alter the levels of Eta-1 expression in the spinal motoneurons. RESULTS： A low level of Eta-1 was found in a subset of spinal motoneurons before avulsion but 3 d after avulsion, the number of Eta-1-expressing motoneurons increased by 52.5%, though the total number of motoneurons reduced by 31.6%. CONCLUSION： Eta-1 is unregulated in spinal motoneurons after spinal root avulsion and it may have some protective effect on motoneurons.
出处
《第四军医大学学报》
北大核心
2005年第18期1642-1644,共3页
Journal of the Fourth Military Medical University
