摘要
目的:探讨舌鳞状细胞癌中肿瘤干细胞对放疗的敏感性。方法:采用磁珠分选技术、细胞免疫荧光染色和细胞增殖活性试验等方法,研究经分选后得到的肿瘤干细胞和阴性细胞在放疗后细胞增殖活性方面的差异。结果:细胞免疫荧光结果显示,CD133和CD44在分选出来的CD133+/CD44+细胞高表达,而在CD133-/CD44-细胞基本不表达。CCK8实验结果显示,分选出来的CD133+/CD44+细胞较CD133-/CD44-细胞在放疗的作用下具有更高的细胞相对增殖率。结论:经磁珠分选出来的舌癌肿瘤干细胞(CD133+/CD44+细胞)更能耐受放疗的作用。
Objective：To investigate the radiosensitivity of cancer stem cells in squamous cell cacinoma of tongue.Methods：CD1 33 ＋/CD44 ＋and CD1 33 -/CD44 -subpopulation cells were sorted by magnetic cell sorting （MACS）.Single isolated CD1 33 ＋/CD44 ＋and CD1 33 -/CD44 -cell was cultured in RPMI-1 640 mediun,which contained 1 0 ng/mL basic fibroblast growth factor （bF-GF）,and 20 ng/mL epidermal growth factor （EGF）.Immunofluorescence was applied to detect the expression of CD1 33 and CD44 in these two subpopulation cells.CCK-8 assay was performed to analyze the radiosensitivity of the two subpopulations.Results：Cell im-munofluorescence showed that CD1 33 and CD44 could be detected in CD1 33 ＋/CD44 ＋ cells while they could not be detected in CD1 33 -/CD44 -cells.Radiation therapy had a more obvious inhibitory effect on CD1 33 -/CD44 - cells than on CD1 33 ＋/CD44 ＋cells.Conclusions：The cancer stem cells in squamous cell carcinoma of tongue（CD1 33 ＋/CD44 ＋cells）that sorted by MACS showed strong capability of tumor′s resistance to radiation therapy than that of CD1 33 -/CD44 -cells.
出处
《口腔生物医学》
2014年第3期125-129,共5页
Oral Biomedicine
基金
广东省科技计划项目(2013-53)
广东省医学科研基金项目(A2014328)
广州市荔湾区科技计划项目(20131215074-1)
关键词
肿瘤干细胞
放疗敏感性
鳞状细胞
肿瘤
Neoplastic stem cells
Radiosensitivity
Squamous cell
Neoplasms
