摘要
本研究采用液体发酵的方法，以发酵液活菌数为指标，采用单因素试验和正交试验对粪肠球菌HEW—A203的培养基和摇瓶发酵条件进行优化。确定最佳培养基的配方为：蔗糖3％，玉米淀粉4％，黄豆粉3％，磷酸氢二钾0．05％，硫酸镁0．1％，MnSO4·H2O 0．025％。最优发酵条件为：接种量2％、初始pH7．0、温度35℃、发酵时间10h、转速180r／min。采用该最优发酵工艺可以降低成本、提高质量，有利于粪肠球菌广泛应用于生产。
This study adopted liquid fermentation method, and use viable count of fermenta- broth as index, by the single factor and orthogonal experiment to optimize culture medium and fermentation conditions of HEW -A203 fermentation. Ultimately to determine the optimal medium composition： 3% sucrose, 4% corn starch, 3% soybean meal, 0.05% dipotassium hydrogen phosphate, 0. 1% magnesium sulfate; optimal fermentation condition is 2% inoculum size, initial pH 7. 0, temperature 35℃, fermentation time 10h, speed of revolution 180r/min. Optimal fer- mentation process could reduce costs, improve quality, and was more conducive to be widely used in production of Enterococcus faecalis.
出处
《中国饲料添加剂》
2014年第9期20-24,共5页
China Feed Additive
关键词
粪肠球菌
正交试验
发酵工艺
Enterococcus faecalis
orthogonal experiment
fermentation process
