摘要
以肇庆七星剑花为材料,通过单因素试验和L9(34)正交试验获得乙醇水浴提取多糖的最佳工艺条件为:提取时间20 min、提取3次、料液比1∶60(g/mL)、乙醇体积分数80%;运用苯酚-硫酸比色法与蒽酮-硫酸比色法测定剑花多糖含量分别为19.80%与20.63%,后者可作剑花多糖的常用测定方法。系统比较了剑花全花、花蕊、花瓣和花萼等不同部位多糖含量的差异,花蕊多糖含量最高,达20.65%;同时应用DPPH法评价该多糖的抗氧化活性(EC50)为5.391μg/mL。
The conditions for ethanol water bath extraction of polysaccharide from Hylocereus undatus （Haw.）Britt. et. Rose were optimized by single facfor test and L9 （3^4） orthogonal array design methods. A material-to-liquid ratio of 1：60 （g/mL） of 80% ethanol for 20 min extractions performed thrice proved to be the optimal. Using phenol sulfuric acid method and anthrone sulfuric acid method for determination of H. undatus polysaecharide content, the contents of polysaceharide were 19.80% and 20.63%. By comparing the content of polysaccharide from stamens, flower, petal and calyx of H. undatus, we found that flower polysaccharide content was the highest, up to 20.65%. And evaluation of the antioxidant activity of the polysaecharide by DPPH method, EC50 was 5.391 μg/mL.
出处
《广东农业科学》
CAS
CSCD
北大核心
2014年第17期101-105,共5页
Guangdong Agricultural Sciences
基金
广东省大学生创新性实验计划项目(1058012034)
肇庆市科技计划项目(2013N007)
