摘要
【目的】构建一张基于PCR技术的谷子分子标记遗传图谱。【方法】以谷子高146A和K103杂交自交F2分离群体为作图群体,以分布于谷子9条染色体上的81个SSR标记为主要参考标记,采用来自谷子、水稻、珍珠粟和高羊茅的SSR、STS、SNP、SV和ACGM标记共1 733个,在亲本间筛选多态性标记,并进一步在F2群体间进行验证,利用MAPMAKER VERSION 3.0软件进行连锁分析,采用MapDraw V2软件绘制遗传连锁图谱。【结果】构建了一张包含192个不同类型分子标记的谷子遗传图谱,新定位标记33个,其中32个来自谷子,另1个来自珍珠粟。遗传图谱包含9个连锁群,覆盖基因组全长2 082.5 cM,连锁群长度介于119.5—475.2 cM,平均长度231.39 cM,标记间平均距离10.85 cM,每个连锁群上的标记数介于10—37个。部分连锁群上标记存在偏分离现象,在定位的192个标记中,共有36个标记发生偏分离,占图谱总标记的18.75%,其中,在LG2、LG6和LG7上分别聚集分布了10、15和7个偏分离标记,出现了偏分离热点区域,而在LG1、LG4、LG5和LG8上仅有零星分布,LG3和LG9上则没有偏分离标记。对来自不同作物的分子标记在谷子上的可转移性分析发现,来自谷子的1 235个PCR标记中有205个标记在双亲及其F2群体间有多态性,多态率为16.60%,而来自珍珠粟、高羊茅和水稻的498个PCR标记,在该群体上仅发现1个多态性标记。【结论】利用不同物种中的分子标记构建了一张覆盖基因组长度为2 082.5cM的谷子遗传连锁图谱,其分子标记主要来自于谷子。
【Objective】 This study aims to construct a genetic map of foxtail millet（Setaria italica（L.） Beauv.） using PCRbased molecular markers. 【Method】 With 81 SSR markers distributed in nine chromosome of foxtail millet as reference markers, a total of 1 733 markers including SSR, STS, SNP, and SV from foxtail millet and ACGM and SSR from pearl millet, tall fescue and rice were used to screen polymorphic markers between Gao 146 A and K103, and the polymorphic markers were tested in the F2 segregation populations derived from cross Gao 146 A / K103. MAPMAKER VERSION 3.0 and MapDraw V2 were used for linkage analysis and integrated genetic map drawing.【Result】A genetic linkage map of foxtail millet containing 192 different kinds of markers was constructed, and 33 markers were newly mapped, of which 32 were from foxtail millet and 1 from pearl millet. The map consisted of nine linkage groups and covered the genome length 2 082.5 cM. Each linkage group was between 119.5-475.2 cM and the average distance of each linkage group spanned 231.39 cM. The number of markers on each linkage group ranged from 10 to 37 and the average distance between different markers was 10.85 cM. There were 36 markers accounted for 18.75% of 192 ones distributed in different linkage group and appeared segregation disorder. There appeared segregation disorder hotspots on LG2, LG6 and LG7, including 10, 15 and 7 segregation markers, respectively, and segregation disorder scattered on LG1, LG4, LG5 and LG8, however, no segregation disorder occurred on LG3 and LG9. According to transferability of molecular markers from different crops, there were 205 polymorphic markers of 1 235 ones from foxtail millet between parents and F2 population and the polymorphism rate was 16.60%, however there was only one polymorphic marker of 498 ones from pearl millet, tall fescue and rice.【Conclusion】A genetic linkage map of foxtail millet, covering the genome length 2 082.5 cM, was constructed using molecular markers from different species and most
出处
《中国农业科学》
CAS
CSCD
北大核心
2014年第17期3492-3500,共9页
Scientia Agricultura Sinica
基金
山西省自然基金(2012011032-1)
国家谷子糜子现代农业产业技术(CARS-07-12.5-A10)
关键词
谷子
分子标记
多态性
遗传图谱
可转移性
foxtail millet
molecular marker
polymorphism
genetic map
transfer ability
