摘要
目的：建立同时测定复方美托洛尔尼群地平胶囊中3种组分（氢氯噻嗪、酒石酸美托洛尔、尼群地平）含量的方法。方法：采用反相高效液相色谱法。色谱柱为Agilent ZORBAX Eclipse XDB—C18，流动相为醋酸盐缓冲液．乙腈，梯度洗脱，流速为2．0ml／min，柱温为30℃，检测波长为275nm。结果：氢氯噻嗪、酒石酸美托洛尔、尼群地平检测质量浓度线性范围分别为45．8-687、92．4～1386、46．1-692μg／ml（r均为0．9999）；平均加样回收率分别为100．27％、100．51％、99．42％，RSD分别为O．69％、1．06％、0．90％（n=6）。结论：该方法简便、准确、重复性好，可为复方美托洛尔尼群地平胶囊质量标准的改进提供参考。
OBJECTIVE ： To establish a method for the content determination of hydrochlorothiazide, metoprolol tartrate and nitrendipine in Compound metoprolol nitrendipine capsules. METHODS： RP-HPLC method was adopted. The determination was performed on Agilent ZORBAX Eclipse XDB-C18 column with mobile phase consisted of acetate buffer-acetonitrile （gradient elution） at the flow rate of 2.0 ml/min. The column temperature was 30 ℃, and the detection wavelength was set at 275 nm. RESULTS： The linear ranges of hydrochlorothiazide, metoprolol tartrate and nitrendipine were 45.8-687 μg/ml （r=0.999 9）, 92.4-1 386 μg/ml （r=0.999 9） and 46.1-692 μg/ml （r=0.999 9）, respectively. The average recoveries were 100.27% （RSD=0.69% , n=6）, 100.51% （RSD=1.06%, n=6） and 99.42% （RSD=0.90%, n=6）, respectively. CONCLUSIONS： The method is sensitive, accurate and reoroducible for the imorovement of aualitv standard of Compound metoorolol nitrendioine cansules.
出处
《中国药房》
CAS
CSCD
2014年第37期3515-3517,共3页
China Pharmacy
