摘要
通过对硫氰酸根离子与铁离子反应得到的硫氰酸铁进行吸光度测定,建立了紫外-可见分光光度法间接测定唾液中硫氰酸根离子含量的分析方法,并对反应酸度、时间、温度进行了研究。在最佳实验条件下,本实验方法的相对标准偏差低于0.89%,质量浓度在0.5--30.0 mg·L-1范围内与吸光度有良好的线性关系（R2=0.999 3）,回收率为90.9%--110.8%。利用该方法测定唾液中硫氰酸根离子的含量取得了满意的效果,而且操作简便,快速准确。
A simple, fast and relatively inexpensive UV -Vis spectrophotometric method for the indirect determination of thiocya-nate ion in saliva , obtained by the absorbance of ferric thiocyanate which was attained by the reaction of thiocyanate ion and ferric ion , is proposed.The influences of acidity , reaction time and temperature were studied .Under the optimized operating conditions , the rela-tive standard deviations were smaller than 0.89%, the linear range of quantification of thiocyanate was 0.5~30.0 mg＆#183; L-1 with the regression coefficient （r2） of 0.9993, and the recovery was found to be 90.9%~110.8%.The present method was successfully ap-plied to the rapid determination of thiocyanate in human saliva.
出处
《西华大学学报（自然科学版）》
CAS
2014年第5期26-29,共4页
Journal of Xihua University:Natural Science Edition
基金
教育部留学回国人员科研启动基金(11233229)
西华大学重点基金项目(Z1023313)
