摘要
目的:研究积雪草皂苷和曲安奈德单独及联合治疗兔耳模型瘢痕的疗效。方法:在20只新西兰兔的耳朵上造成增生性瘢痕,术后第28天开始治疗,新西兰兔分为4组,第1组使用病灶内曲安奈德(TAC)治疗,第2组使用积雪草皂苷治疗,第3组使用两者联合治疗,第4组为控制组。在术后第56天,处死所有新西兰兔,收集伤疤,并使用苏木精-伊红(HE)染色、马松三色染色和CD34免疫组织化学进行处理。结果:各组的ETI如下:组1、2和3,相比对照组,分别下降61.2%、21%和43.3%。SEI下降如下:组1、2和3,相比对照组,分别为40.3%、18.7%和33.3%。马松染色表明,各研究组胶原纤维减少;更细,更有规律。此外,CD34染色表明,各研究组微脉管形成也均减少,检测各组CD34阳性细胞,检测值如下:组1(10.00±7.04),组2(32.5±22.15),组3(20.67±15.83)和组4(43.83±23.95)。结论:TAC比积雪草皂苷和两种药物的联合效果更好,但是两种药物联合比单独使用各个药物表现出更好的胶原排列。
Objective：To investigate the effectiveness of asiaticoside and triamcinolone acetonide in alone and combined treatment of scars in rabbit＇s ear models.Methods：Hypertrophic scars were created on the ears of twenty rabbits.The treatment was started on the 28 th postoperative day.Rabbits were divided into four groups.The first group was treated with intralesional TAC,the second group with asiaticoside,the third group with combination of them.The Fourth group is the control group.On the 56 th day after operation,all rabbits were sacrificed and the scars were harvested and processed with hematoxyline and eosin（HE）staining,Masson＇s Trichrome Stain and CD34 Immunohistochemistry.Results：ETI was decreased 61.2%,21%,and 43.3%for groups 1,2and 3,respectively,compared with control group.The SEI decreased 40.3%,18.7% and 33.3% for groups 1,2and 3,respectively compared with control group.Masson staining showed that the collagen fibers were decreased in all groups,and became thinner and more regular.In addition,CD34 staining indicated that micro vessels formation in the study groups also decreased.CD34 positive cells were detected,and the values were as follows：Group 1（10.00±7.04）,group 2（32.5±22.15）,group 3（20.67±15.83）and group 4（43.83±23.95）.Conclusion：TAC has a better effect than asiaticoside or the combination of both drugs,but the combination regimen shows better collagen arrangement than each drug alone.
出处
《黑龙江医药科学》
2014年第4期20-22,共3页
Heilongjiang Medicine and Pharmacy
关键词
增生性瘢痕
曲安奈德
积雪草皂苷
hypertrophic scars
trimcinolone acetonide
asiaticoside.
