摘要
目的 探讨中国汉族人群中谷胱甘肽过氧化物酶1基因（GPX-1）单核甘酸多态性（SNP）rs3448、rs1050450、rs1800668、m1987628与噪声性听力损失（NIHL）易感性的关联.方法 应用病例-对照研究比较处于同一噪声暴露声压级的作业人员左耳3 000 Hz听阈位移水平,筛选出听阈位移最大的10％个体为本次研究的易感人群,共201例,听阈位移最小的10％个体为本次研究的耐受人群,共202例,针对性进行职业卫生调查和问卷调查.分别抽取两个人群空腹外周静脉血5 ml,常规方法抽提基因组DNA,Taqman探针法化学荧光等位基因鉴别试验检测SNP.结果 GPX1基因SNP rs1987628C等位基因是NIHL的易感基因,与T等位基因相比,OR值为2.531,95％CI为1.878～3.411;CC基因型是NIHL的易感因素,与TT基因型相比,OR值为3.500,95％CI为1.984～6.174.校正混杂因素后,OR值为3.544,95％CI为1.974～6.364.结论 在中国汉族人群中,GPX-1SNP rs1987628位点可能与NIHL易感性有关.
Objective To investigate the association between single nucleotide polymorphisms （SNPs） in glutathione peroxidase 1 （GPX-1） gene,rs3448,rs1050450,rs1800668,and rs1987628,and the susceptibility to noise-induced hearing loss （NIHL） among Chinese Han population.Methods A case-control study was conducted to investigate the threshold shift of the left ear at 3000 Hz among the workers of Chinese Han population who were exposed to the same level of sound pressure.Two hundred and one （10%） of the subjects with the highest level of threshold shift were recruited in susceptible group,while 202 of （10%） of the subjects with the lowest level of threshold shift were recruited in tolerant group.Targeted occupational health survey and questionnaire survey were performed among these people.For each individual,genome DNA was extracted from 5 ml of fasting peripheral venous blood.Four SNPs （GPX-1 rs3448,rs1050450,rs1800668,and rs1987628） were genotyped by the TaqMan SNP genotyping kit.The main effects of SNPs and the association between NIHL susceptibility and SNPs were analyzed by logistic regression.Results The C allele of rs1987628 was a risk factor for NIHL,with an odds ratio （OR） of 2.531 （95%CI：1.878-3.411） as compared with the T allele.The CC genotype of rs1987628 was more associated with NIHL than the TT genotype （OR=3.500,95%CI：1.984-6.174; adjusted OR =3.544,95% CI：1.974 ～6.364）.Conclusion Among Chinese Han population,GPX-1 SNP rs1987628 may be associated with the susceptibility to NIHL.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2014年第8期568-572,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
广东省医学科研基金（A2011048）
