摘要
目的探讨噪声习服对耳蜗抗氧化酶的影响。方法40只雄性豚鼠随机分为无噪声对照组(A组),噪声习服组(B组),噪声习服+强噪声暴露组(C组),单纯强噪声暴露组(D组)。B组动物在声压级为90 dB SPL (声压级)、中心频率为0.5 kHz的一个倍频程噪声下连续暴露10 d,每天6 h;C组动物在经过与B组相同的噪声习服处理后,休息5d,再暴露于105 dB的白噪声4 h;D组动物直接暴露于105 dB的白噪声4 h。噪声暴露结束后即刻测定耳蜗中的脂质过氧化物酶(GSH-Px)活力。结果D组的丙二醛(MDA)水平明显高于其他3组,A、B、C、D组水平分别为144.19±4.59,147.12±4.51,163.76±4.83,187.11±6.14(P<0.05):噪声暴露后C、D组的超氧化物歧化酶(SOD)和GSH-Px活力均有所增强,C组明显高于D组分别为282.20±22.46 vs 243.95±16.53;32.72±2.24 vs 26.84±2.22(P<0.05);D组的过氧化氢酶(CAT)活力明显低于其他3组。而C组的CAT活力增强,A、B、C、D 4组CAT活力分别为63.18±4.59,65.93±4.41,82.34±4.66和45.60±4.88(P<0.05)。结论噪声习服可以提高耳蜗抗氧化酶的活力。
Objective To explore the effect of sound conditioning on antioxidant enzymes of cochlea in guinea pigs. Methods Forty male albino guinea- pigs were equally randomized into 4 groups. The animals were either： （A） kept in quiet cages （control） ; （B） exposed to conditioning noise of a 0. 5 kHz octave band （90 dB for 6 h/day for 10 days） ; （C） exposed to conditioning noise （B） followed by exposure to the higher level noise （105 dB for 4 h） after 5 days＇ rest; or （D） exposed to high level noise （ 105 dB for 4 h） directly. Animals were killed at the end of the experiment, and the cochleas were dissected to determine the levels of malondialdehyde （MDA） and three antioxidant enzymes. Results The level of MDA in group D was significantly higher than those of the other groups （144. 19 ± 4. 59, 147.12 ± 4. 51, 163.76±4. 83, 187. 11± 6. 14 for group A, B, C, D respectively, P 〈0. 05）. After exposure to noise, the activities of superoxide dismutase （SOD） and gluta- thione peroxidase （ GSH - Px） were enhanced, and more significant in group C when compared to group D （282.20 ±22.46 vs 243.95±16.53 ; 32.72±2. 24 vs 26. 84±2. 22 respectively, P 〈 0. 05 ）. High level noise exposure alone decreased catalase activity, while with sound conditioning followed by a brief rest showed an increase in catalase activity over control group （63.18±4.59, 65.93±4.41, 82.34±4.66 and45.60±4.88 for group A, B, C, D respectively, P〈O. 05）. Conclusion The treatment of conditioning noise results in enhancements of antioxidant activity of cochlea.
出处
《中国预防医学杂志》
CAS
2008年第12期1026-1028,共3页
Chinese Preventive Medicine
关键词
噪声习服
耳蜗
丙二醛
抗氧化酶
Sound conditioning
Cochlea
Malondialdehyde
Antioxidant enzymes
