摘要
在批式培养试验中,以牛粪堆肥为天然产氢菌源,以酸解玉米秸秆为底物,通过厌氧发酵生产氢气。利用二因素三水平设计正交实验,考察了初始pH值和初始底物浓度对酸解玉米秸秆发酵产氢的影响,并系统研究了其发酵产氢特性。在初始pH 6.0、底物浓度10g/L的产氢条件下,酸解秸秆的最大产氢量和最大产氢速率分别为265.5mL/(g·TS)和7.9mL/h。通过扫描电子显微镜(SEM)对秸秆形貌进行分析,发现稀酸水解作用可以破坏秸秆的微结构,在发酵产氢过程中混合微生物可直接降解纤维素产氢。
Biohydrogen was produced from aeid--pretreated cornstalk with anaerobic microbes enriched from cow dung compost in batch experiment. The effects of initial pH and substrate concentration on the hydrogen yield were investi- gated by orthogonal experiment design with 2 factors and 3 levels. The maximum hydrogen yield of 265.5 mL/（g·TS） and hydrogen production rate of 7.9 mL/h were obtained under the condition of I0 g/L substrate concentration and initial pH 6.0. The scanning electron microscopy （SEM） was used to study the changes in the cornstalk composition during the treatment of chemical--microbial process. It was shown that the acid pretreatment of the cornstalk plays a key role in the conversion of cornstalk into biohydrogen and the mixture microorganisms can directly degrade the cellulose to produce hydrogen.
出处
《郑州牧业工程高等专科学校学报》
2014年第2期1-3,7,共4页
Journal of Zhengzhou College of Animal Husbandry Engineering
基金
国家自然科学基金河南省人才培养联合基金项目(U1204522)
河南省高校科技创新人才支持计划项目(13HASTIT033)
河南牧业经济学院科研创新团队资助
关键词
玉米秸秆
发酵产氢
优化
发酵特性
cornstalk
hydrogen fermentation
optimization
characteristic
