摘要
目的:比较不同干燥方式对祖师麻有效成分含量的影响。方法:采用HPLC测定祖师麻甲素和伞形花内酯、紫丁香苷含量,流动相均为乙腈-0.1%甲酸水(15∶85),检测波长依次为326,265 nm;利用UV测定总香豆素含量,检测波长326 nm。通过单因素试验考察自然晒干、自然阴干、烘箱干燥、真空干燥对祖师麻中祖师麻甲素、伞形花内酯、紫丁香苷和总香豆素含量的影响。结果:不同干燥方式对祖师麻甲素质量分数的影响顺序为真空干燥>80℃烘干>40℃烘干>50℃烘干>自然阴干>60℃烘干>105℃烘干>自然晒干,伞形花内酯为80℃烘干>自然阴干>真空干燥>105℃烘干>50℃烘干>60℃烘干>40℃烘干>自然晒干,紫丁香苷为40℃烘干>自然阴干>自然晒干>真空干燥≈50℃烘干>60℃烘干>80℃烘干>105℃烘干,总香豆素为真空干燥>自然阴干>50℃烘干>自然晒干>60℃烘干>80℃烘干>40℃烘干>105℃烘干。结论:真空干燥能最大程度地保存各有效成分,其次为自然阴干和50℃烘干。建议产地加工生产时采用自然阴干;若产地或饮片厂家条件允许,建议采用35℃真空干燥,其次选择50℃烘干。
Objective：To compare effects of different drying methods on contents of active components in Cortex Daphnes.Method：HPLC was adopted to determine contents of syringin,daphnetin and umbelliferone with mobile phrase of acetonitrile-0.1％ formic acid （15∶ 85） and detection wavelength at 265,326 nm,respectively.The content of total coumarins was determined by UV at a wavelength of 326 nm.Effects of different drying methods on contents of active components in Cortex Daphnes were investigated by single factor test.Result：The content line of daphnetin by different drying methods was vacuum drying ＞ oven drying at 80 ℃ ＞ oven drying at 40 ℃ ＞ oven drying at 50 ℃ ＞ drying in the shade ＞ oven drying at 60 ℃ ＞ oven drying at 105 ℃ ＞ drying in the sun,the content line of umbelliferone was oven drying at 80 ℃ ＞ drying in the shade ＞ vacuum drying ＞ oven drying at 105 ℃ ＞ oven drying at 50 ℃ ＞ oven drying at 60 ℃ ＞ oven drying at 40 ℃ ＞ drying in the sun,the content line of syringin was oven drying at 40 ℃ ＞ drying in the shade ＞ drying in the sun ＞ vacuum drying≈ oven drying at 50 ℃ ＞ oven drying at 60 ℃ ＞ oven drying at 80 ℃ ＞ oven drying at 105 ℃,the content line of total coumarins was vacuum drying ＞ drying in the shade ＞ oven drying at 50 ℃ ＞ drying in the sun ＞ oven drying at 60 ℃ ＞ oven drying at 80 ℃ ＞ oven drying at 40 ℃ ＞ oven drying at 105 ℃.Conclusion：Vacuum drying could maximally preserve active components,followed by natural drying in the shade and oven drying at 50 ℃.It recommended natural drying in the shade when processing in production place; if conditions allowed in origin or pieces manufacturers,vacuum drying at 35 ℃ was the best method to dry Cortex Daphnes,followed by oven drying at 50 ℃.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第16期48-50,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
济南市高校院所自主创新科技计划项目(201102017)
