摘要
采用平板划线法从纳豆中分离了一株具有γ-聚谷氨酸(γ-polyglutamicacid,γ-PGA)生产能力的菌株(JPND618),通过对该菌株形态、生理生化特性研究以及16S rRNA序列对比分析,对其进行了鉴定。同时对发酵产物进行了分析鉴定,研究了其吸水性能、阻垢性能、絮凝性能及可生物降解性能。结果表明,该菌株生化特征与芽孢杆菌属相似,且其16S rDNA序列与Bacillus amyloliquefaciens NBRC 15535同源性达98%,确定该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。发酵生产γ-PGA产量为4.86g/L,发酵产物γ-PGA的吸水性、可生物降解性明显优于聚丙烯酰胺(PAM),保水性能与PAM相当,对CaSO4、CaCO3的阻垢率最高可达70%以上,对高岭土悬浮液具有良好的絮凝作用,表明菌株JPND618发酵生产γ-PGA是一种性能优良的环境友好型生物材料,具有良好的应用前景。本研究为γ-PGA的发酵生产提供了一株新的菌种,对γ-PGA的生物合成研究及应用具有积极意义。
A γ-polyglutamicacid（γ-PGA） producing strain（JPND618） was isolated using streaked plate method. The strain was identified through strain morphological, physiological-biochemical characteristics and sequence contrastive analysis of 16 S rRNA. The fermenting product was identified, and its water absorption property, scale property, flocculation property and biodegradable property were studied. The results showed that, the physiological-biochemical characteristics of strain JPND618 were similar to bacillus genus, and the homelogy of 16 S rDNA sequence with Bacillus amyloliquefaciens NBRC 15535 reached 98%, thus strain JPND618 was identified as Bacillus amyloliquefaciens. The yield of γ-PGA was 4.86 g/L. γ-PGA showed much better water absorption and biodegradable property than Polyacrylamide（PAM）, and similar water-retaining property with PAM. The anti scale rates towards CaSO4, CaCO3 reached more than 70%, and flocculation towards Gaolin suspension was good. γ-PGA produced by strain JPND618 was proved as a kind of environmental friendly biological materials with high performance, which has a better application prospect. This study supplies a new strain for producing γ-PGA.
出处
《现代食品科技》
EI
CAS
北大核心
2014年第7期44-50,共7页
Modern Food Science and Technology
基金
陕西省科技厅自然基金项目(2012JM2005)
2012年地方高校国家级大学生创新创业训练计划项目(201210708030)
关键词
Γ-聚谷氨酸
菌种分离鉴定
解淀粉芽孢杆菌
产物性能
γ-polyglutamicacid
strain isolation and identification
Bacillus amyloliquefaciens
product properties
