摘要
从土壤中筛选分离获得一株γ-聚谷氨酸合成菌PGS-1,经鉴定为枯草芽孢杆菌(Bacillus subtilis),在富含谷氨酸和葡萄糖的培养基中可大量合成γ-聚谷氨酸,摇瓶发酵产量达26 g/L,不同于大多文献报道的微生物合成的γ-聚谷氨酸具有较高的分子量,该菌株合成的γ-聚谷氨酸分子量较低(3×105-4×105 kD),分子量分布较窄,可适用于低分子量要求的应用领域,如作为药物的控缓释载体,值得深入开发研究。
A novel γ-polyglutamic acid(γ-PGA) producing-strain Bacillus subtilis PGS-1 was isolated from soil,which could produced a large mount of γ-PGA in the medium containing glucose and glutamate.The highest yield 26 g/L of γ-PGA was obtained in flask culture.Compared with other reported γ-PGA producing-strain,γ-PGA derived from Bacillus subtilis PGS-1 had a lower molecular weight(3×105-4×105 kD) and a narrower polydispersity(1.3-2.0),which could endow γ-PGA a promising application,such as in the drug delivery system.
出处
《微生物学通报》
CAS
CSCD
北大核心
2011年第2期164-168,共5页
Microbiology China
基金
江苏省高校自然科学研究项目(No.09KJB530007)
