摘要
目的 :探讨多种细胞因子诱导的杀伤细胞(cytokine induced killer,CIK)联合紫杉醇对卵巢癌SKOV-3细胞的体外杀伤效应。方法 :应用多种细胞因子在体外将健康志愿者外周血单核细胞诱导成CIK细胞,收获培养第14天的CIK细胞作为效应细胞,FCM法分析其表型特征。应用CIK细胞培养上清液培养SKOV-3细胞24和48 h后,FCM法检测SKOV-3细胞的凋亡情况。MTT法检测不同效靶比CIK细胞、不同浓度紫杉醇和紫杉醇(2.5μg/mL)联合不同效靶比CIK细胞(分别为10∶1和20∶1)作用后SKOV-3细胞的增殖抑制率。结果:CIK细胞体外培养第14天时,CD3+CD56+细胞所占比例为(35.43±2.38)%。CIK细胞培养上清液培养SKOV-3细胞后,细胞凋亡率明显高于对照组(未用CIK细胞培养上清液干预)(P<0.01)。CIK细胞和紫杉醇对SKOV-3细胞的抑制率随着效靶比和药物浓度的增加及作用时间的延长而增强(P<0.05)。CIK细胞联合紫杉醇(2.5μg/mL)干预48 h后,SKOV-3细胞的抑制率明显高于各单独应用组(P<0.01)。结论 :CIK细胞可能通过诱导卵巢癌SKOV-3细胞的凋亡而发挥细胞杀伤作用。联合CIK细胞可明显增强紫杉醇对卵巢癌SKOV-3细胞的杀伤作用。
Objective： To investigate the anti-tumor effect of cytokine-induced killer （CIK） cells combined with paclitaxel against ovarian cancer SKOV-3 cells in vitro. Methods： Peripheral blood mononuclear cells of healthy volunteers were stimulated by different cytokines and induced into CIK cells （as effector cells）. The phenotype of CIK cells after culture for 14 d was analyzed by flow cytometry （FCM）. The apoptosis of SKOV-3 cells cultured with CIK cells’ supernatant for 24 and 48 h was analyzed by FCM. The proliferative inhibition rates of SKOV-3 cells after treatment with CIK cells with different effector-target ratios or different concentrations of paclitaxel and paclitaxel （2.5 μg/mL） in combination with CIK cells with different effector-target ratios （10︰1 or 20︰1） were detected by MTT assay. Results： The proportion of CD3＋CD56＋ cells in CIK cells was （35.43±2.38） % after culture for 14 d. The apoptosis rate of SKOV-3 cells after culture with CIK cells’ supernatant was higher than that of the control cells （without culture with CIK cells’ supernatant） （P 〈 0.01）. The inhibition rate of SKOV-3 cells was increased with the increase in effector-target ratio of CIK cells and the concentration of paclitaxel as well as with the prolonged culture time （P 〈 0.05）. The inhibition rate of SKOV-3 cells treated with combination of CIK and paclitaxel （2.5 μg/mL） for 48 h was higher than those treated with CIK and paclitaxel alone （both P 〈 0.01）. Conclusion： CIK cells have strong anti-cancer activity against ovarian cancer SKOV-3 cells by inducing apoptosis. It can enhance the anti-tumour effect of paclitaxel against SKOV-3 cells when in combination with CIK cells.
出处
《肿瘤》
CAS
CSCD
北大核心
2014年第7期591-595,共5页
Tumor
基金
国家自然科学基金资助项目(编号:30950022)
徐州市医学科研项目(编号:XWJ2011038)
