摘要
采用微波辅助提取法提取海带脂肪酸，并利用酸、碱两种不同酯化法对其进行甲酯化，同时结合气相色谱-质谱联用（GC-MS）技术分析其脂肪酸组成。结果共检测出20种脂肪酸，酸化甲酯化法检测出17种，碱化甲酯化法检测出13种，其中不饱和脂肪酸含量分别为53.60%和54.87%，主要为ω-6族和ω-3族的亚油酸（16.57%~19.95%）、花生四烯酸（3.57%~3.81%）、γ-亚麻酸（2.12%~2.40%）、十八烷四烯酸（2.45%~3.38%）和二十烷五烯酸（1.99%~2.39%）。棕榈酸是主要的饱和脂肪酸，其含量分别占脂肪酸总量的30.46%和22.19%。与文献报道相比，运用微波辅助提取，结合酸、碱两种甲酯化法的GC-MS分析方法更能全面分析海带中所含脂肪酸成分。所得结果可为海带这一丰富资源的开发利用提供参考。
In this study, the fatty acid compositions in Laminaria japonica were extracted by microwave-assisted extraction method （MAE）, and their compositions were analyzed by GC-MS combining acid-catalyzed and base-catalyzed methyl esterification methods. The results revealed that 20 kinds of fatty acid compositions were identified, of which 17 were detected in acid-catalyzed methyl esterification condition, and 13 kinds of fatty acid compositions were determined using base -catalyzed methyl esterification method. The main fatty acid compositions were unsaturated fatty acids, which accounted for 53.60%of total fatty acids in acid-catalyzed method and 54.87 % in base-catalyzed method. The same unsaturated fatty acid compounds detected in two catalytic methods were linoleic acid （16.57%-19.95%）, arachidonic acid （3.57%-3.81%）,γ-linolenic acid （2.12%-2.40%）, stearidonate （2.45%-3.38%） and eicosapentaenoate （1.99%-2.39%）. In addition, palmitic acid detected in two catalytic methods above was the main saturated fatty acid composition of Laminaria japonica. The relative percentage content of palmitic acid was 30.46 % and 22.19 %, respectively. Compared with conventional method, this model employing GC-MS technology coupled with MAE and acid/base-catalyzed methyl esterifications methods in the analyses of the fatty acid compounds from Laminaria japonica can evaluate the fatty acid compositions more completely. The results obtained could be helpful for the exploitation and utilization of Laminaria japonica.
出处
《食品研究与开发》
CAS
北大核心
2014年第14期83-86,共4页
Food Research and Development
基金
广州市科技计划项目(12A62011558)
广州市科技计划项目(12C32011650)
广州市番禺区科技计划项目(2010-专-12-08)
关键词
微波提取
海带
脂肪酸
甲酯化
GC—MS
microwave-assisted extraction
Laminariajaponica
fatty acid
methyl esterification
GC-MS
