摘要
目的探讨狼疮肾炎(LN)外周血OX40,OX40L mRNA表达水平及抗C1q抗体水平。方法采集80例系统性红斑狼疮患者及40例正常对照者外周血,采用ELISA法检测抗C1q抗体,采用荧光定量逆转录聚合酶链反应(RT-PCR)测定外周血单个核细胞(PBMC)中OX40,OX40L mRNA表达水平。结果LN患者抗C1q抗体水平为(9.11±2.46)ng/mL,显著高于非LN患者的(3.12±0.97)ng/mL及正常对照者的(1.12±0.33)ng/mL,差异有统计学意义(P<0.05)。LN患者PBMCs OX40,OX40L mRNA水平分别为(8±4)×106拷贝/μL和(4±3)×103拷贝/μL,显著高于非LN患者[(4±3)×103及(2±2)×103]和正常对照者[(4±2)×103及(2±1)×103],差异有统计学意义(P<0.01)。结论外周血OX40,OX40L mRNA表达及抗C1q抗体与LN活动度相关,其在LN的发生、发展中可能起一定作用。
Objective To investigate Clq antibody level and the expression of costimulatory molecules OX40 and OX40L messenger RNA （mRNA） in peripheral blood of patients with lupus nephritis（LN）. Methods Pe- ripheral blood was collected from 80 patients with SLE and 40 normal human controls. The OX40 and OX40L mRNA were determined in peripheral blood mononuclear cells （PBMCs） by fluorescence quantita- tive reverse transcription polymerase chain reaction （FQ-RT-PCR）. The Clq antibody was detected by ELISA. Results The levels of OX40 / OX40L mRNA and Clq antibody were significantly increased in pa- tients with LN [ （8±4） ×106copy/p.L, （4 ±3） ×103 copy/μL, （9. 11 ±2.46）ng/mL] than those of SLE patients without LN [ （4 ±3） ×103, （2 ±2） ×103, （3. 12 ±0. 97）ng/mL] and normal controls[ （4 ± 2） ×103, （2± 1 ）×103 , （ 1. 12 ± 0.33 ） ng/mL] （ P 〈 0.05 ）. Conclusion Upregulation of costimulatory molecules OX40, OX40L mRNA and Clq antibody in peripheral blood may play a certain role in genesis and progression of LN.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2014年第8期792-793,822,共3页
The Chinese Journal of Dermatovenereology
基金
天津市卫生局课题(2012KZ062)
