摘要
目的：建立牛蒡根药材的质量标准。方法：采用薄层色谱（TLC）法对牛蒡根中的齐墩果酸进行定性鉴别；对药材的水分、总灰分、浸出物进行测定。采用高效液相色谱（HPLC）法测定牛蒡根药材中绿原酸的含量：色谱柱为Alltima C18（250mm×4．6mm，5μm），流动相为乙腈．0．4％磷酸水溶液（13：87，V/V），流速为1ml／min，柱温为30℃，检测波长为327nm。结果：TLC鉴别中，供试品在对照品、对照药材色谱相应位置上显示相同颜色的荧光斑点；对药材水分、总灰分、浸出物含量进行了限定；绿原酸进样量在0．1408～1．0560μg范围内与峰面积积分值呈良好的线性关系（r=0．9999），平均加样回收率为99．48％（RSD=1．34％，n=6）。结论：该方法专属性强，能准确进行定性和定量检测，可用于牛蒡根药材的质量控制。
OBJECTIVE：To investigate the quality standard ofArctium lappa. METHODS： TLC was used to qualitatively identify oleanolic acid in A. lappa. The contents of moisture, total ash, extract were determined. HPLC was used to determine the content of chlorogenic acid in A. lappa. The determination was performed on Alltima C18（250 mm×4.6mm,5μm） column with mobile phase consisted of acetonitrile-0.4% phosphoric acid （13：87, V/V） at the flow rate of 1 ml/min. The detection wavelength was set at 327 nm, and column temperature was 30℃. RESULTS ： The test samples had same color TLC spots to substance control and reference substance on the same position. The contents of moisture, total ash and extract in A. lappa were defined. The linear range of chlorogenic acid were 0.140 8-1.056 0 μg（r=0.999 9） with an average recovery of 99.48% （RSD=1.34% ,n=6）. CONCLUSIONS： The method is specific and can be used for qualitative detection and quantitative detection. It also can be used for the quality control ofA. lappa.
出处
《中国药房》
CAS
CSCD
2014年第31期2934-2936,共3页
China Pharmacy
基金
山东省科技发展计划项目(No.2010GNC10924)
青岛市民生计划项目(No.13-1-3-99-nsh)
