摘要
为了研究多个SNPs共同作用对肉质产生的作用,采用PCR-SSCP技术首次对杂种及纯种山猪的MYF5的全部外显子进行多态性分析,并利用SPSS statistics 16.0分析单个SNP及SNPs组成的单倍型对部分肉质性状的影响。结果表明:在第1个外显子内检测到2个SNPs,即955 bp处的A→C的突变,形成2种基因型(AA型和AC型),1 038 bp处的G→C的突变,形成2种基因型(GG型和GC型),2个突变位点均未发现纯合的突变型;A/C突变位点对蛋白质影响显著(P<0.05);A/C和G/C突变位点单独对剪切力的影响均不显著(P>0.05),组成的单倍型M-S(2个位点均突变)对剪切力影响显著(P<0.05),表明2个突变位点的互作会产生出新的遗传效应。3种单倍型AG、A-S和M-S在纯种山猪与杂种山猪中的分布存在明显差异;M-S单倍型不仅对蛋白质的影响显著(P<0.05),对剪切力的影响亦显著(P<0.05)。
In order to study the possible effects of multiple SNPs on meat quality, the PCR-SSCP was used to investigate different bloodlines of Shanzhu populations to find the polymorphisms of the total three exons of MYF5gene for the first time , and SPSS statistics 16.0 was used to analyze the association of MYF5 aflelic variants and haplotypes with some production traits. Result showed that a total of two SNPs on exon 1 were found. The first SNP was A→C in 955 bp which form two genotyoes（AA and AC）; The second SNP was G--~C in 1 038 bpwhich form two genotypes （GG and GC）, both of the mutations were not found homozygous mutant-type alleles. A/C allele significantly influence protein content （P〈0.05） but G/C allele don＇t（ P〉0.05）; Individual A/C or G/C allele had no significant effect on the shear force （P〉0.05）, however, M-S type （both sites are mutated） had significant influence on shear force （P〈O.05）, which mean interaction of two mutations will be generate new genetic effects. The distribution of three haplotypes A- G, A-S and M-S had significant difference in different bloodlines of Shanzhupopulations （P〈0.05）; M-S type not only significantly influenced protein content but also shear force （P〈0.05）. The study results provided an important theoretical reference for the molecular marker assisted breeding of pigs.
出处
《中国畜牧杂志》
CAS
北大核心
2014年第15期19-23,共5页
Chinese Journal of Animal Science
基金
江苏省自然科学基金(BK2009402)
