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CBG基因外显子Ⅱ SNPs对猪背膘厚和肌肉颜色的影响

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摘要 本试验采用PCR—SSCP检测方法研究猪CBG基因外显子Ⅱ在金华×皮特兰F2资源家系猪群中的DNA多态性,发现了3种基因型(GGCC、GGCG和AAGG)。对纯合子个体测序检测,发现在CBG基因第二外显子内存在2个多态位点(G149A和G157C),其中G149A位点的单核苷酸替换导致CBG基因相应位置编码的氨基酸发生变化,由缬氨酸(Val)变为异亮氨酸(Ⅱe)。对金华×皮特兰F2资源家系猪进行了该多态片段的基因型鉴定,并分析了不同基因型对猪背膘厚和肌肉颜色的遗传效应,结果显示:GGCC基因型猪的6—7肋骨背膘厚以及a^*值分别比AAGG基因型猪的高0.31cm和0.73cm,差异显著(P〈0.05);GGCC基因型猪的b^*值分别比AAGG基因型和GGCG基因型猪的高0.66和0.93,差异显著(P〈0.05);GGCG基因型猪的c^*值分别比GGCC基因型和AAGG基因型猪的高6.57和7.40,差异显著(P〈0.05);GGCC基因型猪的h^*值比AAGG基因型猪的低4.58,差异显著(P〈0.05)。
出处 《江苏农业科学》 CSCD 北大核心 2009年第1期201-203,共3页 Jiangsu Agricultural Sciences
基金 湖北省教育厅优秀中青年人才项目(编号:Q200626001)
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