摘要
目的观察临床常用的静脉麻醉药咪达唑仑对离体完整海马锥体神经元自发放电活动的影响，探讨全身麻醉药的作用机制。方法将20只体重为30-40g的幼年Sprague-Dawley大鼠经异氟烷吸入麻醉后迅速断头取脑，剥出其完整的海马组织。应用离体膜片钳技术记录海马CA1区锥体神经元自发放电活动。灌流浓度为10μmol／L的咪达唑仑，观察其对海马CA1区锥体神经元动作电位发放频率的影响。结果剥出的幼年大鼠完整海马组织能够存活并具有自发放电的特性。离体海马CA1区锥体神经元动作电位的发放频率为（0．605±0．366）Hz，灌流咪达唑仑后显著降低至（0．266±0．153）Hz（P〈0．05）。结论10μmol／L咪达唑仑能明显抑制海马CA1区锥体神经元自发放电活动。
Objective To investigate the effect of midazolam, an intravenous anesthetic, on the spontaneous discharge of pyramidal cells in OA1 area of intact,hippocampus in vitro, so as to provide evidence for the mechanism of action of general anesthetic. Methods After anesthetized with isoflurane, 20 neonatal Sprague- Dawley rats weighing 30-40 g were decapitated. Their brains were removed and the hippocampal formation was dissected. Spontaneous discharge of pyramidal ceils in CA1 area was recorded by patch clamp in vitro. Midazolam 10 μmol/L was perfused and the effect of midazolam on the rate of discharge of action potential of hippocampus was observed. Results CA1 pyramidal cells in dissected intact hippocampus could keep alive and discharge spontaneously. The action potential frequency of pyramidal cells of in-vitro hippocampus was （0. 605 ± 0.366） Hz, which was decreased significantly after perfusing midazolam （0. 266±0. 153） Hz （P〈0. 05）. Conclusion Spontaneous discharges of CA1 pyramidal cells can be significantly depressed by 10 μmol/L midazolam.
出处
《上海医学》
CAS
CSCD
北大核心
2014年第6期461-463,F0003,共4页
Shanghai Medical Journal
关键词
海马
膜片钳
咪达唑仑
锥体细胞
动作电位
Hippocampus
Patch clamp
Midazolam
Pyramidal cells
Action potential
