摘要
目的观察脑出血大鼠微创术后骨髓间充质干细胞（BMSCs）动员对神经功能的影响。方法将45只大鼠建立脑出血模型后，随机分为两组，对照组（n=20，行微创引流手术）和治疗组（n=25，微创引流手术后3～5d开始BMSCs动员）。术前当天及术后2、4、8周行Garcia神经功能量表评分，术前当天、术后2周测定外周血CD133^＋、CD34^＋细胞数，术前及术后2、4、8周行肝肾功能检查。结果两组术后Garcia量表分值均显著高于术前（P〈0．05），治疗组术后各时间点Garcia神经功能量表分值显著高于对照组（P〈0．05）。术后2周，治疗组外周血CD133^＋、CD34^＋细胞在单核细胞（MNCs）中的比例明显高于对照组（P〈0．05）。BMSCs动员后肝肾功能检查指标均正常。结论大鼠脑出血微创术后早期进行BMSCs动员，没有肝肾功能损伤，并可促进神经功能恢复。
Objective To observe the impact of autologous bone marrow mesenchymal stem cells （BMSCs） mobilization on neurological function recovery after cerebral hemorrhage minimally invasive surgery in rats. Methods After experimental intracerebral hemorrhage models was established, 45 SD rats were divided into two groups randomly, control group rats （20 rats, minimal invasive hematoma aspiration after modeling） and treated group （25 rats, begin BMSCs mobilization after aspiration 3-5 days）. Garcia scales were performed at pre-modeling and 1, 4, 8 weeks post aspiration, CD133＋, CD34＋ cell counts were measured in peripheral blood at pre-modeling and 2 weeks post aspiration, liver and renal function were performed in each group at 1, 3 months post BMSCs mobilization. Results Postoperative Garcia scores were significantly higher than those preoperative （P〈0.05） in two groups, postoperative Garcia scores in treated group were significantly higher （P〈0.05） than those in control group. Two weeks postoperation, the proportion of CD133＋, CD34＋ cells in mononuclear cells in peripheral blood of treated group was significantly higher than those in control group （P〈0.05）. 1, 3 months postoperative liver and renal function were normal. Conclusion After minimally invasive surgery of cerebral hemorrhage, BMSCs mobilization as early as possible can performed without adverse reactions, and can promote the recovery of neurological fimction.
出处
《中华临床医师杂志（电子版）》
CAS
2014年第14期95-98,共4页
Chinese Journal of Clinicians(Electronic Edition)
基金
江西省科技计划项目(20112BBG70079)
关键词
脑出血
外科手术
微创性
造血干细胞动员
神经功能修复
Cerebral hemorrhage
Surgical procedures, minimally invasive
Hematopoietic stem cell mobilization
Neurological functional recovery
