摘要
以不同生育期转cry1 Ac+cry2Ab基因棉和常规棉田土壤为对象,采用传统平板培养、Biolog分析和变性梯度凝胶电泳(PCR-DGGE)技术,研究了该新型转基因材料对土壤细菌群落结构和功能多样性的影响。可培养细菌数量测定结果表明,在棉花整个生育期,2种棉田土壤可培养细菌数量具有相同的变化趋势;与常规棉相比,转cry1 Ac+cry2 Ab基因棉田可培养土壤细菌数量在蕾期存在显著差异(P<0.05),其他生育期则无显著差异。Biolog分析结果显示,转cry1 Ac+cry2 Ab基因棉和常规棉田土壤细菌活性和碳源利用能力在苗期和蕾期存在明显差异,其他生育期则无明显不同。PCR-DGGE图谱的聚类分析结果表明,转cry1 Ac+cry2 Ab基因棉与常规棉田土壤细菌群落结构均随着生育期的不同而发生改变,且两者仅在花铃期出现明显差异。对PCR-DGGE条带的序列分析表明,转cry1 Ac+cry2 Ab基因棉与常规棉田土壤细菌之间的差异具有随机性,可能与采样时期或采样点的差异有关。综上所述,2种棉田土壤细菌群落结构和功能多样性在整个棉花生育期的变化趋势基本一致,转cry1Ac+cry2Ab基因棉仅在某些时期对其产生短暂影响,大部分时期无明显影响。
Soil samples were collected from cotton fields of transgenic cry1 Ac＋ cry2 Ab cotton and conventional cotton ateach growth stage for analysis of soil bacteria and 16S rDNA gene, using the methods of conventional plate culture, Biologanalysis and polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） analysis, and impacts of thetransgenic cotton on bacterial community structure and functional diversity of soil bacteria have been discussed. Determina-tion of the population of culturable bacteria indicates that the soils in the two cotton fields shared a similar trend in varia-tion of the population of culturable bacteria throughout the entire cotton growth period, and no significant difference existedbetween the two in total population of soil bacteria, except at the budding stage（P〈0. 05）. Biolog analysis shows that thesoil bacteria in the two fields differed significantly in activity and carbon utilization capacity at the seedling and bud stages（P〈0. 05）. Cluster analysis of the PCR-DGGE profiles of the soil bacteria demonstrated that soil bacteria in both fieldsvaried in community structure with growth stage and differed sharply only at the flower and boll stage. PCR-DGGE band se-quencing analysis shows that the differences between soil bacteria of the two fields were quite random, which may be attrib-uted to differences in sampling timing and sampling site. To sum up, soil bacteria in the two cotton fields shared a similartrend in variation of community structure and functional diversity throughout the entire growth period, and the impact oftransgenic cotton was short and temporary at certain growth stages, and nill during most of the growth period.
出处
《生态与农村环境学报》
CAS
CSCD
北大核心
2014年第2期206-213,共8页
Journal of Ecology and Rural Environment
基金
转基因生物新品种培育重大专项(2013ZX08012-005
2013ZX08011-002)
国家科技支撑计划(2013BAD11B01)
