摘要
目的研究人牙周膜干细胞与牙周膜非干细胞之间差异表达的microRNA(miRNA)的靶基因并对其功能进行预测分析。方法本研究于2011年7月至2013年10月在福建医科大学附属口腔医院进行。采用基因芯片技术筛选人牙周膜干细胞与牙周膜非干细胞之间差异性表达的miRNA,并通过生物信息学对差异表达的miRNA进行靶基因预测及靶基因功能富集分析。结果与人牙周膜非干细胞相比,人牙周膜干细胞中明显上调的关键miRNA 25个,调控的关键靶基因21个;明显下调的关键miRNA 5个,调控的关键靶基因9个。结论特异性表达的miRNA主要调控的靶基因及其基因功能与人牙周膜干细胞的干性维持密切相关。
Objective To study the mechanism of action and the regulatory network between the specific expression of mieroRNA （miRNA）and the target gene function in human periodontal ligament stem ceils and non-stem cells. Methods miRNA mieroarray was used to detect differential expression of miRNA between human periodontal ligament stein cells and non-stem cells. The way of biological information was used to predict the targets gene and gene function of these miRNAs, and to analyze the functional enrichment of target genes. Results Twenty-five miRNA were obviously up-regulated in human periodontal ligament stem cells and 21 key target genes were predicted. Five miRNA were obviously down-regulated in human periodontal ligament stem cells and 9 key target genes were predicted. Conclusion The tar- get genes and gene function which are regulated by specific miRNA are closely related to the sternness of human peri- odontal ligament stem cells.
出处
《中国实用口腔科杂志》
CAS
2014年第7期403-406,共4页
Chinese Journal of Practical Stomatology
基金
福建省自然科学基金面上项目(2011J01169)
福建省科技厅重点项目(2011Y0024)
