摘要
Purpose:.To examine the relationship between corneal inflammation and corneal lymphangiogenesis after keratoplasty.Methods:.Rat corneal lymphangiogenesis was examined by lymphatic vessel endothelial receptor(LYVE-1) immunohistochemistry and whole mount immunofluorescence at 1, 3, 7,10, and 14 days after corneal transplantation. Corneal inflammation was evaluated by inflammation index(IF) grading and NF-κB immunohistochemistry at the same time points. The association between lymphatic vessel counting(LVC) and the IF scores was then examined.Results:.LYVE-1 positive lymphatic vessels occurred in the corneal stroma on day 3,.developed throughout days 7 and10,.and peaked in number at day 14 after keratoplasty.Corneal inflammation was strong on day 3, and then resolved gradually,.but increased again from days 7 to 14 after the transplantation..LVC was strongly and positively correlated with IF after keratoplasty(r=0.41;P<0.05). However, changes in IF scores and LVC were not parallel.Conclusion:.A close,.but not parallel,.relationship was found between corneal lymphangiogenesis and corneal inflammation after corneal transplantation.
Purpose： To examine the relationship between corneal inflammation and corneal lymphangiogenesis after keratoplasty.
Methods： Rat corneal lymphangiogenesis was examined by lymphatic vessel endothelial receptor （LYVE-1） immunohistochemistry and whole mount immunofluorescence at 1, 3, 7, 10, and 14 days after corneal transplantation. Corneal inflammation was evaluated by inflammation index （IF） grading and NF-κB immunohistochemistry at the same time points. The association between lymphatic vessel counting （LVC） and the IF scores was then examined.
Results： LYVE-1 positive lymphatic vessels occurred in the corneal stroma on day 3, developed throughout days 7 and 10, and peaked in number at day 14 after keratoplasty. Corneal inflammation was strong on day 3, and then resolved gradually, but increased again from days 7 to 14 after the transplantation. LVC was strongly and positively correlated with IF after keratoplasty（r=0.41 ;P〈0.05）. However, changes in IF scores and LVC were not parallel.
Conclusion： A close, but not parallel, relationship was found between corneal lymphangiogenesis and corneal inflammation after corneal transplantation.
基金
supported by China Natural Science Foundation(81070711)
Guangdong Provincial Science and Technology Projects(2012B031800456)
Doctoral Program of the Ministry of Education(20110171120104)
the Young Teachers Training Program of Sun Yat-sen University(11ykpy42)
