摘要
以2,4-二羟基水杨醛为原料经Knoevenagel缩合反应、脱乙酰化反应,再与4-二乙氨基水杨醛经缩合反应,进而通过甲基化合成了新型的香豆素荧光探针.利用核磁共振氢谱(1 H NMR)、碳谱(13 C NMR)和高分辩质谱对所合成的目标化合物进行了结构确认,并测定了其荧光光谱.结果表明,目标化合物对Cu2+有较好的选择性和灵敏度,且当Cu2+浓度在0.66~10μmol·L-1范围内时探针的荧光强度与其浓度有较好的线性关系.Job分析法表明探针与金属离子作用形成1∶1结合的络合物.
A novel coumarin-based fluorescent probe was synthesized through Knoevenagel condensation,deacelation reaction,condensation with 4-diethylamino salicylaldehyde and methylation with 2,4-dihydroxy salicylaldehyde as the starting material.The structure of the target compound was confirmed by nuclear magnetic resonance spectrometry(^1 H NMR,^13C NMR)and high resolution mass spectrometry,and its fluorescence spectrum was measured.Results demonstrate that the probe exhibits high selectivity and sensitivity towards Cu^2+.There exists agood linear correlation between the fluorescence intensity of probe and Cu^2+concentration in the range of 0.66-10μmol·L^-1.In addition,the Job′s plot with fluorescence titration further confirms that the probe interacts with Cu^2+via1∶1binding forming a complex.
出处
《化学研究》
CAS
2014年第4期345-348,358,共5页
Chemical Research
基金
国家自然科学基金(21272056)
河南省科技厅基础与前沿项目(112300413224)
河南省教育厅自然科学项目(2010B350002)
关键词
香豆素
铜离子
荧光探针
合成
性能
coumarin
Cu^2+
fluorescence probe
synthesis
properties