摘要
目的观察丹红注射液对脂多糖(LPS)诱导的THP-1巨噬细胞促炎因子分泌的影响,并探讨其机制。方法 160 nmol/L佛波酯孵育THP-1巨噬细胞24 h后分为对照组、脂多糖组、丹红组和脂多糖+丹红组,酶联免疫吸附法检测培养液中促炎因子的含量,Western blot检测核因子κB(NF-κB)、p65和Toll样受体4(TLR4)的蛋白表达水平。结果丹红注射液明显抑制LPS诱导的巨噬细胞肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和白细胞介素1β(IL-1β)释放,下调核因子NF-κB和TLR4的表达。结论丹红注射液抑制脂多糖诱导的炎症反应,可能与其抑制TLR4和NF-κB的表达有关。
Objective This study was to investigate the effect of Danhong injection on proinflammatory cytokine release from lipopolysaccharide （LPS）-induced THP-1 macrophages. Methods Human THP-1 macrophages were induced using phorbol-12-myristate acetate （PMA, 160 nmol/L） for 24 h, then cells were divided into four groups：control group, LPS group, Danhong group and LPS ＋ Danhong group. Secretion of proinflammatory cytokines,including tumor necrosis factor-α （ TNF-α）, interleukin-6 （ IL-6 ） and interleukin-1β （ IL-1β ） was performed by enzyme-linked immunosorbent assay （ELISA）. The protein expression of nuclear factor-κB （NF-κB） p65 and Toll-like receptor 4 （TLR4） were examined by Western blot. Results Danhong injection inhibited the release of TNF-α, IL-6 and IL-1β induced by LPS, repressed the translocation of NF-κB to the nucleus and TLR4 expression. Conclusions Danhong injection inhibited the release of inflammatory cytokine that induced by LPS, and the mechanisms may be related to the TLR4 and NF-κB expressions.
出处
《中南医学科学杂志》
CAS
2014年第5期443-446,共4页
Medical Science Journal of Central South China
基金
衡阳市科技局项目资助(2103K159)
