摘要
目的:探讨不同胆固醇含量结石的微生物群落差异.方法:应用末端标记限制性片段长度多态性(terminal restriction fragment length polymorphism,T-RFLP)和克隆文库分析,以微生物群落16S rRNA基因(16S rDNA)为目标,对62例细菌常规培养为阴性的胆囊结石患者中的结石成分及微生物群落结构进行了解析和比较.结果:(1)根据结石所含胆固醇的不同,分为胆固醇结石组(胆固醇含量≥90%)11例、胆色素结石组(胆固醇含量<70%)26例和混合性结石组(胆固醇含量70%-89%)25例;(2)胆固醇组结石表面细菌16S rDNA阳性率为18.1%(2/11),结石核心细菌16S rDNA阳性率为24.3%(3/11).胆色素组结石表面细菌16S rDNA阳性率为42.3%(11/26),结石核心细菌16S rDNA阳性率为53.8%(14/26).混合性结石组结石表面细菌16S rDNA阳性率为36.0%(9/25),结石核心细菌16S rDNA阳性率为64.0%(16/25).结论:胆固醇含量不同的结石中优势菌有所差别,但以肠杆菌科和微球菌科为主.其中肠杆菌科(Enterobacteriaceac)的沙门氏菌属(Salmonella)伤寒沙门氏菌可能在结石形成过程中有重要作用.
AIM： To analyze the gallstone composition and microbial community structure in gallstones. METHODS： The composition of gallstones and microbial community structure in gallstones from patients with cholecystolithiasis were investigated by terminal restriction fragment length polymorphism and clone library approaches. Small subunit rRNA gene （16S rDNA） was analyzed in 62 patients with cholecystolithiasis and negative bile culture RESULTS： According to the percentage of cholesterol, the 62 patients with cholecystolithiasis were divided into three groups： 11 in the cholesterol group （cholesterol content ≥90%）, 26 in the pigment group （cholesterol content 〈 70%） and 25 in the mixed group （cholesterol content between 70% and 89%）. The positive rates of 16S rDNA on stone surface in the cholesterol group, pigment group and mixed group were 18.1% （2/11）, 42.3% （11/26） and 36.0% （9/25）, respectively. The positive rates of 16S rDNA in stone core in the cholesterol group, pigment group and mixed group were 24.3% （3/11）, 53.8% （14/26） and 64.0% （16/25）, respectively. CONCLUSION： The structure of the bacterial community is different in gallstones with different cholesterol contents.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第17期2467-2472,共6页
World Chinese Journal of Digestology
基金
云南省应用基础研究计划重点基金资助项目
No.2006C008Z~~
